FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_26.3510000004167c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_26.3510000004167c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345238
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC148964.314704638539211RNA PCR Primer, Index 13 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGT53341.545021115867894No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT19870.5755449863572376No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT12540.36322768640763764No Hit
TCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCTCC9730.2818345605060857No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA9250.2679311083947885No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCG9250.2679311083947885No Hit
CTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCTCC9050.26213800334841475No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT8140.2357793753874139No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCT7200.20855178166945704No Hit
CACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCT6890.19957246884757762No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC5580.1616276307938292No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACCTGTCTCTTA5420.15699314675673015No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC5390.15612418099977407No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGACTGTCTCTTATAC4920.14251038414079562No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTT4810.13932417636529004No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACTGTCTCTTAT4140.11991727445993779No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTC4140.11991727445993779No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT3600.10427589083472852No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC3560.10311726982545374No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT3520.101958648816179No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCTGTCTCTTATACACA3460.10022071730226684No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGT207.012501E-445.0181161
TGCCGTC15950.044.71642745
CGGGTAT301.13848386E-437.4987917
CGGGGCG250.002103906836.004064
GTCATAC250.002105403535.9988449
CAGACGG250.002105403535.9988449
ATGCCGT20750.034.37238344
TATGCCG21800.032.7168443
CGTATGC21900.032.6701841
ATCTCGT22000.032.52168337
TCGTATG22000.032.52168340
AATCTCG22000.032.4194136
CTCGTAT22100.032.37452339
GTATGCC22050.032.34589842
CGAGACC22050.032.2438623
CACGAGA22200.032.2286921
CCACGAG22200.032.2286920
CCCACGA22250.032.15626519
CGGGGAC352.8144554E-432.1464774
GTCTGCG352.8168492E-432.14182321
Produced by FastQC (version 0.11.2)