FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_25.35100000041653.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_25.35100000041653.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143478
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC15811.101911094383808RNA PCR Primer, Index 13 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGT5370.3742734077698323No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC2090.14566693151563306No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC1820.126848715482513No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT1770.12336386066156484No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT1740.12127294776899594No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT1720.11987900584061667No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC1630.11360626716291No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT1610.11221232523453073No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA1600.1115153542703411No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCG1520.10593958655682405No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT1450.10106078980749661No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA206.991239E-445.02793
TGCCGTC2700.040.83017345
GAGTGCT301.13546645E-437.4970937
TACGGCC250.002101778835.997218
GTATTAA458.587758E-735.0217062
TATTATT551.1099837E-732.7475662
AGAGTGC352.8036992E-432.1515776
TCCCCGC352.8094446E-432.1403723
ATGCCGT3550.031.05393244
CTATTCA3800.030.1950329
ACGGGAT300.005116885530.01863
AGGGTGG300.005125623730.0081356
GGGGGTT300.005125623730.0081355
TACCGGG300.00513437429.99767521
AGACCTA3800.029.6029725
CGTATGC3800.029.01091241
ACCTATT3900.028.84391827
CGAGACC3900.028.84391823
GACCTAT3900.028.84391826
TATGCCG3850.028.63414643
Produced by FastQC (version 0.11.2)