FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_24.35100000041639.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_24.35100000041639.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127018
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC13521.0644160670141238No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCA6070.4778850241698027No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC5610.44166968461162986No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTC3520.27712607661906186No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGT3470.27318962666708657No Hit
GTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTC3100.24405989702246927No Hit
GGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGG3090.2432726070320742No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT2990.23539970712812358No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATG2720.21414287738745688No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGA2710.2133555873970618No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGA2600.20469539750271618No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT2430.19131146766600007No Hit
GCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCTG2330.18343856776204948No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCC2270.1787148278196791No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC2260.17792753782928403No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT2250.177140247838889No Hit
GTATCAACGCAGAGTACGGGCATCCTCCAAGGCTAAATACTCCTGACTGAC2240.17635295784849392No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA2230.17556566785809885No Hit
GTCTGGGCCTTCCCACATCGTTTCCCACTTAACCATGACTTTGGGACCTTA2190.1724165078965186No Hit
CCATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACG2180.17162921790612354No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCA2010.15824528806940746No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTC1940.15273425813664207No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGCCC1920.15115967815585193No Hit
GTATCAACGCAGAGTACGGGGCGGTCCGGCCGGGAACTCAAAGGAGACTGC1920.15115967815585193No Hit
CATCCTGGCTGTCTGGGCCTTCCCACATCGTTTCCCACTTAACCATGACTT1910.15037238816545687No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG1890.14879780818466676No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA1870.14722322820387662No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCG1840.14486135823269142No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTG1830.14407406824229638No Hit
TATCAACGCAGAGTACGGGGCGGTCCGGCCGGGAACTCAAAGGAGACTGCC1800.14171219827111117No Hit
ACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCA1790.1409249082807161No Hit
CTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCG1740.1369884583287408No Hit
GGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAA1740.1369884583287408No Hit
TGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTT1730.13620116833834575No Hit
GTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA1730.13620116833834575No Hit
CTTCTAAGCCAACATCCTGGCTGTCTGGGCCTTCCCACATCGTTTCCCACT1720.13541387834795068No Hit
TATCAACGCAGAGTACGGGCATCCTCCAAGGCTAAATACTCCTGACTGACC1690.13305200837676548No Hit
CCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTG1680.13226471838637044No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC1660.1306901384055803No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCC1630.12832826843439513No Hit
CTTCAGCACCGTAGTGCCTCGTCATCACGCCTCAGCCTTGATTTTCCGGAT1590.12517910847281488No Hit
GGTATCAACGCAGAGTACGGGGCGGTCCGGCCGGGAACTCAAAGGAGACTG1580.12439181848241981No Hit
GTCCCAAAGTCATGGTTAAGTGGGAAACGATGTGGGAAGGCCCAGACAGCC1580.12439181848241981No Hit
TTACTCACCCGTCCGCCACTCGTCAGCAAAGAAGCAAGCTTCTTCCTGTTA1530.1204553685304445No Hit
GGTATCAACGCAGAGTACGGGCATCCTCCAAGGCTAAATACTCCTGACTGA1500.11809349855925932No Hit
TTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCC1480.11651891857846919No Hit
GTACGGGGCGGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTG1460.11494433859767907No Hit
ACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGG1440.11336975861688894No Hit
TCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCTGACCACCT1390.10943330866491363No Hit
CGTTAGCACCCGCCGTGTGTCTCCCGTGATAACATTCTCCGGTATTCGCAG1370.1078587286841235No Hit
CTTCACGACGGACGTTAGCACCCGCCGTGTGTCTCCCGTGATAACATTCTC1340.10549685871293832No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCAC1330.10470956872254326No Hit
GTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGATT1330.10470956872254326No Hit
CAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGCCTT1320.1039222787321482No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGCTGTCTC1300.10234769875135809No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1280.10077311877056795No Hit
GATTTGATGTTACCTGATGCTTAGAGGCTTTTCCTGGAAGCAGGGCATTTG1280.10077311877056795No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1550.042.0960445
GACCGTT650.041.5377357
TAGGACC700.038.5707554
GGACCGT900.037.4993446
TATCGTT301.13433074E-437.49934413
GTTATCG301.13433074E-437.49934411
GGTATCA5750.036.4193421
TCGTTAC250.002100254435.9993715
CGTTACG250.002100254435.9993716
TTAGGAC751.8189894E-1235.9993673
AGGACCG751.8189894E-1235.9993675
ATGCCGT1900.034.34150344
CTTAGGA801.8189894E-1233.749412
TTATCGT352.8066523E-432.14229612
TATTGTT352.8066523E-432.14229613
CGTTATT352.8066523E-432.14229610
TGTTACG352.8066523E-432.14229616
TTATTGT352.8066523E-432.14229612
GTTATTG352.8066523E-432.14229611
GCCTAGT501.95155E-631.5242711
Produced by FastQC (version 0.11.2)