FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_23.3510000004161f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_23.3510000004161f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158858
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC149559.414067909705523No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGT133398.39680721147188No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCG107256.751312492918203No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC7590.477785191806519No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA5490.3455916604766521No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA4210.2650165556660665No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT3890.24487277946342018No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCC3630.22850596129876996No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA3610.22724697528610457No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC3140.19766080398846772No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA2780.17499905576049052No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGC2660.16744513968449812No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC2560.1611502096211711No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC2430.152966800538846No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC2250.14163592642485742No Hit
TGTCTTATGTCTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATC2210.13911795439952662No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG1900.11960367120321294No Hit
TGTCTTATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTT1830.11519722015888402No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT1810.11393823414621865No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC1800.11330874113988593No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA1770.11142026212088783No Hit
GATAAGACACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTT1630.10260736003223005No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC1620.10197786702589734No Hit
TGTCTTATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTGTC1600.10071888101323195No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG207.0149224E-445.08
GGAATAA253.875592E-544.99999631
GCACTCG253.875592E-544.99999621
TGCCGTC16650.043.37837645
GCTGTCA501.0695658E-940.49999628
TGTACGG501.0695658E-940.49999620
CCCCTCC301.1354985E-437.50000429
CACTCGA301.1354985E-437.50000422
ACTCGAA301.1354985E-437.50000423
TGCACTC301.1354985E-437.50000420
CTATGGT250.002101658635.9999963
GATCGTA250.002101658635.9999961
CATGATA250.002101658635.99999628
CGCTGTA250.002101658635.99999613
TACGCTG250.002101658635.99999611
CACCAGC250.002101658635.99999631
GTGTAAT458.6373257E-735.01
TGTCACC606.357368E-933.75000430
CTGTCAC606.357368E-933.75000429
TTGTGTA606.357368E-933.75000417
Produced by FastQC (version 0.11.2)