FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_21.351000000415cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_21.351000000415cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255149
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC110934.347655683541774No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGT99233.889100094454613No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCG45711.7915022202712925No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA30991.2145844193000954No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT17220.6748997644513598No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA10110.3962390603137775No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT6770.26533515710428024No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGA6750.26455130139643895No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC6490.254361177194502No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA6350.24887418723961294No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA6150.24103563016119992No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCA6120.239859846599438No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTTATACAC5300.20772176257794467No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC4800.18812536988191214No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC4410.1728401835790068No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC4410.1728401835790068No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT4410.1728401835790068No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT4400.17244825572508612No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC4090.16029849225354598No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATACA3900.1528518630290536No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCTCTTATAC3870.15167607946729167No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATC3840.1505002959055297No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC3730.14618908951240256No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTGTCTCTTATACA3430.13443125389478305No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTCTGTCTCTTATACACATCTC3320.1301200475016559No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT3220.1262007689624494No Hit
AACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCC3190.12502498540068746No Hit
AACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGA3170.12424112969284613No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGC3120.12228149042324289No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACC3090.12110570686148094No Hit
GGGAAACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTA2930.11483486119875054No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGCTGTCTCTTATACAC2800.10973979909778209No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTC2800.10973979909778209No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTCTGTCTC2790.10934787124386143No Hit
AAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATACACAT2780.10895594338994079No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTCTGTCTCTTATACACAT2770.10856401553602014No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC2770.10856401553602014No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA2750.10778015982817882No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA2750.10778015982817882No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC2580.10111738631152778No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10750.042.48596645
TTACACA250.002100580836.0120774
GTCGACT250.002104628435.9979634
CCGTCTT504.855974E-835.9979617
CTGAAAC4200.032.67672344
GTCTACA300.005131413730.0100672
ACCCCAT300.005131413730.0100676
AAGCCAT300.00514126429.99830220
ATCCCCT602.4077053E-729.99830226
CGATGCT300.00514126429.99830226
ATCGATT300.00514126429.99830225
GTACTTA1650.028.6515881
ATTCAGC554.15009E-628.63474345
TAGATAA406.140828E-428.1344385
GGGGGTT406.140828E-428.1344385
TGAAACA4550.027.19626245
GTACTGG2600.026.8411881
TGGTTCA2750.026.1906035
GGTTCAC2750.026.1906036
ACTGAAA5350.025.65275243
Produced by FastQC (version 0.11.2)