FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_20.351000000415a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_20.351000000415a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284101
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC28701.0102041175497445No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC23160.8152030439878775No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT21090.7423416320252304No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGT16140.5681078208102048No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT14070.4952464088475578No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA11380.4005617720458569No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC10450.36782693478727635No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC10250.36078718483919453No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC7800.2745502479751919No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA7480.26328664805826096No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT7410.2608227355764323No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG7270.25589491061277503No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT7200.2534309981309464No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCT6160.21682429840092077No Hit
CACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCT5570.19605703605407934No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTT5550.19535306105927117No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA5480.19288914857744252No Hit
CTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCTCC5170.18197753615791568No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCG5100.179513623676087No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC5010.1763457361994502No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGACTGTCTCTTATAC4840.17036194874358063No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC4800.16895399875396427No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACCTGTCTCTTA4740.16684207376953972No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACTGTCTCTTAT4520.15909834882664967No Hit
TCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCTCC4470.1573384113396292No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTC4460.1569864238422251No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCT4460.1569864238422251No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC4450.15663443634482102No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCA4230.148890711401931No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC4180.14713077391491056No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA4140.14572282392529418No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGG3920.13797909898240415No Hit
TCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATAC3910.13762711148500004No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGT3880.13657114899278777No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT3840.1351631990031714No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC3650.12847543655249366No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT3460.1217876741018159No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG3450.12143568660441181No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT3390.11932376161998726No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACG3340.1175638241329668No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACT3300.11615587414335042No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA3250.11439593665632997No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT3240.11404394915892586No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT3160.11122804917969313No Hit
CTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC2910.10242836174459084No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCG207.026208E-444.99753613
CACACTC406.7866495E-944.99753641
TGCCGTC3800.039.07680545
CTATAGT356.22651E-638.5761074
TGTCGAC356.22651E-638.57610733
AGTCCGG301.1381267E-437.49794818
ATTCGCA301.1381267E-437.49794815
CATTCGC301.1381267E-437.49794814
GTTACTA250.002103192736.0043643
TCGCACT250.00210501135.9980317
CACTCTG504.8592483E-835.9980343
CGCACTT250.00210501135.9980318
CGACTCC250.00210501135.9980341
GGACGTA352.8130665E-432.14675527
TGCTCAG300.00513776630.0036375
TCTGACC300.005142190529.9983642
CCGTCTT300.005142190529.9983617
AGTGCGT300.005142190529.998368
CGGATTG300.005142190529.9983622
GTCGTAG300.005142190529.9983613
Produced by FastQC (version 0.11.2)