FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_15.35100000041503.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_15.35100000041503.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410576
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC245265.973559097463077No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGT188524.59159814504501No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT28020.6824558668797007No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCG20470.49856786563267214No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC15580.3794668952885702No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC8680.21141031136744476No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT8550.20824402790226415No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT8390.20434706363742644No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA7720.1880285257784186No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCT7380.1797474767156385No Hit
CACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCT7350.17901679591598146No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACCTGTCTCTTA7250.1765811932504579No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTT6960.16951794552043958No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC6730.1639160593897354No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA6650.16196757725731656No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA6580.16026265539145007No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC6040.14711040099762285No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC5980.14564903939830873No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA5850.1424827559331281No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG5820.14175207513347102No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA5760.1402907135341569No Hit
CTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCTCC5750.14004715326760453No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGACTGTCTCTTATAC5440.1324967850044815No Hit
TCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCTCC5270.12835626047309145No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCT5120.12470285647480613No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT4810.11715248821168309No Hit
ACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACA4560.11106348154787421No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC4500.10960211994856008No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTGCG351.2091368E-745.00109521
TGCCGTC26450.044.57032845
CGTCTGC356.2374384E-638.57236520
TCGTCTG356.2374384E-638.57236519
AATGCGT301.139297E-437.4963535
GCAAGCC250.002105282436.00087724
CTAGGAC250.002105282436.00087726
CGTTTAG401.5578737E-533.75082426
TCACGCA352.812813E-432.151474
TTCAACG352.812813E-432.151472
CGTTAAC352.818852E-432.13972539
GGTGTTC300.00514283630.00073230
GGGCGCA300.00514283630.00073220
TTATGGG300.005145929.99707812
CGGTATA300.005145929.99707836
GCTGTGC300.005145929.9970788
TAACCTA14300.029.7457315
AACCTAT14750.028.8382366
ACCTATG15300.027.9384547
CCTATGG15300.027.791418
Produced by FastQC (version 0.11.2)