FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_14.351000000414f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_14.351000000414f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534888
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC73971.3829063280537233No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGT35170.6575208267899074No Hit
TTGTAGTACTTGGCTGATTCTTTCTCATAACGTGTGAGGTTATTTTTGAAA8580.16040741239287476No Hit
TTGTAATACTTGGCTGATTCTTTCTCATAACGTGTGAGGTTATTTTTGAAA8130.1519944362184233No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCG6740.12600768759067318No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8300.040.11731345
CACCGAC401.5598558E-533.7473347
GACTCCC1050.029.9976337
TAACCTA3300.028.6501715
ATGCCGT12150.027.59041444
TTAGACT1800.026.2626574
ACTCCCA1200.026.2479278
TATGCCG12950.025.88598643
CGAGACA13400.025.68826923
CACGAGA13600.025.6413621
ACGAGAC13600.025.47593122
CGTATGC13450.025.42550341
CCACGAG13450.025.42550320
GTATGCC13300.025.20477742
TCGTATG13600.025.14507540
CTCGTAT13550.025.07182139
CCCACGA13650.025.05296919
ATGGGTG450.001222256525.0120545
CGGTTCC450.001226261524.99802830
GCCCACG13700.024.96153318
Produced by FastQC (version 0.11.2)