FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_10.3510000004147e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_10.3510000004147e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67168
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC16022.3850643163411145No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC2480.3692234397332063No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGT2460.3662458313482611No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA1090.16227965697951405No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC1070.15930204859456884No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC1030.15334683182467843No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCG870.12952596474511674No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC870.12952596474511674No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG820.1220819437827537No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT740.11017151024297284No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1900.040.2631645
CGTATGC1950.039.2307741
ATAGACA1950.039.2307729
CCCACGA1950.039.2307719
TCGTATG1950.039.2307740
CGAGACA1950.039.2307723
ACATAGA1950.039.2307727
AATCTCG1900.039.0789536
TAGACAA2000.038.2530
GACAAAT2000.038.2532
CACGAGA2000.038.2521
GAGACAT2000.038.2524
CATAGAC2000.038.2528
AGACATA2000.038.2525
AGACAAA2000.038.2531
CTCGTAT1950.038.07692339
TCTCGTA1950.038.07692338
CCACGAG2050.037.31707420
ACAAATC2000.037.12533
GCCCACG2100.036.4285718