FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pa_1.35100000041356.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pa_1.35100000041356.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346903
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC71002.046681637230004No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGT10710.3087318357004696No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC9880.28480583909623153No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA4640.13375496896827066No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC4420.12741313854305092No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC3890.11213509251865796No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC3830.11040550240268893No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG3680.10608152711276639No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTGC207.026582E-444.9998553
TGCCGTC8550.037.368345
TCGTATG8650.036.936340
GCGTTAT8600.036.88941632
CTCGTAT8700.036.72402239
CGAGACA8700.036.72402223
CGTATGC8750.036.51416841
TATCTCG8850.036.35581636
ATGCCGT8800.036.306744
CGTTATC8850.036.10157833
GTATGCC8850.035.84734342
TATGCCG8900.035.64595443
TGCGTTA8900.035.64595431
TCTCGTA9000.035.49988638
AATGCGT9050.035.05513429
AGACAAT9300.034.83859625
CACGAGA9200.034.7281521
CCCACGA9300.034.59666419
GAGACAA9300.034.3547324
ATGCGTT9250.034.29718830