FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_96.35100000047353.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_96.35100000047353.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTC910.0No Hit
TTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTCT55.555555555555555No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGTC22.2222222222222223RNA PCR Primer, Index 33 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTCTTC22.2222222222222223RNA PCR Primer, Index 45 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGTC22.2222222222222223No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA22.2222222222222223No Hit
ATAAAGCCCGAATACATACACTCCGATATAACTGGAAAAATTTTTTCTGTG11.1111111111111112No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCTTTAATAC11.1111111111111112No Hit
GGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATC11.1111111111111112No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGATTTATCTCGTATGCCGTC11.1111111111111112TruSeq Adapter, Index 2 (95% over 21bp)
ATGCATATAAAAGCCAAAGAATGCCAATGATTGCCAAGAGACAAAGATAGA11.1111111111111112No Hit
AAGCACAGGCCCAGCATGCCGATGAAGGCCTGTCTCTTATACACATCTCCG11.1111111111111112No Hit
CCCGTTCACAATGCTGCCCCGCGCGATATGGGCAAACAGATGCAGTAGCAT11.1111111111111112No Hit
ACGCAGAGTACTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTGCTTTGATAC11.1111111111111112No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA11.1111111111111112No Hit
AACCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCCAGGCC11.1111111111111112No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.1111111111111112No Hit
GTACGGGACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.1111111111111112No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCGTC11.1111111111111112No Hit
GTTGATACCACTGCCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACAC11.1111111111111112No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGT11.1111111111111112No Hit
GAGTACGGCAGCAGTGGTATCAACGCAGAGTACGGAAGCAGCGGTATCAAC11.1111111111111112No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAC11.1111111111111112No Hit
GTACGGGAAGCAAGTGGTATCAACGCAAAAAAAAACAAAAAAAAAAAAAAA11.1111111111111112No Hit
GGATTGGAAAAACTGTAGCAAAGGAAATATGTGGATTCTTAGAGCAAATAC11.1111111111111112No Hit
TATCAACGCAGAGTACGGGGTGGTATCAAGCAGCTGTCTCTTATACACATC11.1111111111111112No Hit
GTCACACCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATT11.1111111111111112No Hit
TATCAACGCAGAGTACGGGAGTGGTATCAACGCAGAGTACTTTCTGTCTCT11.1111111111111112No Hit
TAATAACCCCGACTATGGGGTGGATGATGGCGCCGTAGCTACTCAACATGC11.1111111111111112No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCC11.1111111111111112No Hit
CGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTAC11.1111111111111112No Hit
GGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGAAAAA11.1111111111111112Illumina PCR Primer Index 11 (96% over 30bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTATA11.1111111111111112No Hit
TTATACACATCTCCGAGCCCACGAGACCTAGCTTCATCTCGTATGCCGTCT11.1111111111111112Illumina PCR Primer Index 11 (95% over 21bp)
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTATTTTTTTTTTT11.1111111111111112No Hit
TGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA11.1111111111111112No Hit
TTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGTCT11.1111111111111112RNA PCR Primer, Index 33 (100% over 21bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGACTCC11.1111111111111112No Hit
GTGGTATCAACGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCGTCCT11.1111111111111112No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.1111111111111112No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTGGTGCCCCCGTTCCC11.1111111111111112No Hit
CATTAGGCGTCGCGGCCAGGAGGTGACGTTCTGATGCTGTCTCTTATACAC11.1111111111111112No Hit
GTGGTATCAACGCAGAGTACGGGGGTATCAACGCAAAAAAACTGTCTCTTA11.1111111111111112No Hit
CTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTC11.1111111111111112No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA11.1111111111111112No Hit
GTACGGGTGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.1111111111111112No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAAAAAAA11.1111111111111112No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.1111111111111112No Hit
GGCTCGGAGATGTGTATAAGAGACAGCCGCATACCCCCGGCAAGAAGACGC11.1111111111111112No Hit
CTTATACACATCTCCGAACCCACGAGACCTAGCGTTATCTCGTATTCCGTC11.1111111111111112No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.1111111111111112No Hit
ATCAACGCAGAGTACGGGGGTATCAACGCAGAGTACTTTTTAAAAAAAAAA11.1111111111111112No Hit
GGCGCATACCAGTAATCTCAGCTACTCGGGCTGTCCCTTATACACATCTCC11.1111111111111112No Hit
TGCTGGAGCAGGGCGGCTTCCCCGCCGCCTGAAGCTGACGACCGGCCAAGT11.1111111111111112No Hit
CGGGTGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.1111111111111112No Hit
GTACTTTTTTTTTTTTTTTTTTGCGTTTATACAACCCCTGTCACTTATACA11.1111111111111112No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCCCGTATGCCGTC11.1111111111111112No Hit
TCCACGTAGTGTTGGGCCTGCGGGTGCACAGAAGGCAAAAGTTGGGGTTCG11.1111111111111112No Hit
GCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCC11.1111111111111112No Hit
ATTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTC11.1111111111111112No Hit
GTCTTAGCGTCATGGAGTGGTCGCAAATGTGGAATTGTTGAACCGTCCACT11.1111111111111112No Hit
GCGTTGATACCACCCGTACTCTGTGTTGATACCTGTCTCTTATACACATCT11.1111111111111112No Hit
AGTACTACCCGCGCCTGAAGCAGGAGGCCTGGAGCTGTCTCTTATACACAT11.1111111111111112No Hit
CTTGATTACTGGTCAAGACTTAGCCTTATGTTTGGATGGTTTGACATTTTT11.1111111111111112No Hit
CCACACCACCTATGCATTCAGTAGTGGGCGACCACACATAACCGTGGCCAG11.1111111111111112No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGA11.1111111111111112No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT11.1111111111111112No Hit
TCCTCTACGATCTTGCTTTTCTCGCTAACATAGATACCATCAAGAAACTTC11.1111111111111112No Hit
CTTATACACATCTCCGAGCCCACGATCTCGTATGCCGTCTTCTGCTTGAAA11.1111111111111112Illumina PCR Primer Index 1 (96% over 29bp)
GTTACCGTACATGGCGAACATGCAGGCAACGATCATGAGGGCGATACCCAG11.1111111111111112No Hit
CTTATACACATCTCCGAGCCCACGAACGGCATAGGATCTCGTATGCCGTCT11.1111111111111112No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGGTTCAACTCGTATGCCGTC11.1111111111111112No Hit
GCCCAGGAATTTGAGACCAGCTTGGGCAACATAGCAAGACCCCATCTCAAA11.1111111111111112No Hit
CCTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTC11.1111111111111112RNA PCR Primer, Index 45 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC50.045.017
TAGCGAT50.045.032
GTATGCC50.045.042
ATCTCGT50.045.037
ATCTCCG50.045.010
CACATCT50.045.07
CACATAG50.045.028
GCCCACG50.045.018
TATACAC50.045.03
TGCCGTC50.045.045
GCGATCT50.045.034
CCACGAG50.045.020
TATGCCG50.045.043
CATCTCC50.045.09
GAGCCCA50.045.016
CACGAGA50.045.021
CCCACGA50.045.019
ACATCTC50.045.08
TTATACA50.045.02
TACACAT50.045.05
Produced by FastQC (version 0.11.2)