FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_95.35100000047339.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_95.35100000047339.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73814
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTC2321331.447963800904976No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGT19272.6106158723277426No Hit
TTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTCT7731.0472268133416425No Hit
ATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTCTTC5030.681442544774704TruSeq Adapter, Index 7 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCG4560.6177689869130518No Hit
TATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTCTT2010.27230606659983203RNA PCR Primer, Index 7 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC25550.040.9491245
CGTCTTC750.039.045
ATGCCGT27550.037.97640644
CGAGCCC28000.037.76785715
AGCCCAC28000.037.687517
CCCACGA28000.037.687519
CCGAGCC28200.037.65957314
CTAGGTT28050.037.54010829
GACCTAG28050.037.54010826
TCCGAGC28300.037.526513
TATGCCG27900.037.543
GGTTCAT27900.037.532
GCCCACG28150.037.4866818
CACGAGA28150.037.4866821
GAGCCCA28150.037.4866816
AGACCTA28100.037.4733125
ACGAGAC28100.037.4733122
CCTAGGT28050.037.45989628
TAGGTTC28000.037.44642630
ATCTCGT27900.037.41935737