FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_91.351000000472a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_91.351000000472a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56100
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTC1795532.00534759358289No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGT15752.807486631016043No Hit
TTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTCT5390.9607843137254901No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCG3960.7058823529411765No Hit
ATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTCTTC3760.6702317290552585TruSeq Adapter, Index 7 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTCTT1420.2531194295900178RNA PCR Primer, Index 7 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC20950.042.83420645
ATGCCGT22650.039.5199844
TCCGAGC23500.038.4733513
AGCCCAC23400.038.4455417
CCTAGGT23200.038.42344328
CTCCGAG23550.038.3916712
CGAGCCC23500.038.37764715
ACCTAGG23350.038.36942327
GAGCCCA23450.038.36356716
TCTCGTA23300.038.35515238
TAGGTTC23250.038.34081330
CTAGGTT23250.038.34081329
GAGACCT23400.038.2874424
GACCTAG23400.038.2874426
AGGTTCA23300.038.25853731
CGTATGC23400.038.25331541
TATGCCG23400.038.25331543
GTATGCC23400.038.25331542
GCCCACG23500.038.22030318
CACGAGA23500.038.22030321