Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0120_pe_91.351000000472a4.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 56100 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTC | 17955 | 32.00534759358289 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGT | 1575 | 2.807486631016043 | No Hit |
TTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTCT | 539 | 0.9607843137254901 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCG | 396 | 0.7058823529411765 | No Hit |
ATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTCTTC | 376 | 0.6702317290552585 | TruSeq Adapter, Index 7 (95% over 22bp) |
TATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTCTT | 142 | 0.2531194295900178 | RNA PCR Primer, Index 7 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 2095 | 0.0 | 42.834206 | 45 |
ATGCCGT | 2265 | 0.0 | 39.51998 | 44 |
TCCGAGC | 2350 | 0.0 | 38.47335 | 13 |
AGCCCAC | 2340 | 0.0 | 38.44554 | 17 |
CCTAGGT | 2320 | 0.0 | 38.423443 | 28 |
CTCCGAG | 2355 | 0.0 | 38.39167 | 12 |
CGAGCCC | 2350 | 0.0 | 38.377647 | 15 |
ACCTAGG | 2335 | 0.0 | 38.369423 | 27 |
GAGCCCA | 2345 | 0.0 | 38.363567 | 16 |
TCTCGTA | 2330 | 0.0 | 38.355152 | 38 |
TAGGTTC | 2325 | 0.0 | 38.340813 | 30 |
CTAGGTT | 2325 | 0.0 | 38.340813 | 29 |
GAGACCT | 2340 | 0.0 | 38.28744 | 24 |
GACCTAG | 2340 | 0.0 | 38.28744 | 26 |
AGGTTCA | 2330 | 0.0 | 38.258537 | 31 |
CGTATGC | 2340 | 0.0 | 38.253315 | 41 |
TATGCCG | 2340 | 0.0 | 38.253315 | 43 |
GTATGCC | 2340 | 0.0 | 38.253315 | 42 |
GCCCACG | 2350 | 0.0 | 38.220303 | 18 |
CACGAGA | 2350 | 0.0 | 38.220303 | 21 |