FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_9.35100000046851.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_9.35100000046851.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13675
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCGTC354425.915904936014627TruSeq Adapter, Index 11 (95% over 24bp)
TCTTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCGT2742.003656307129799TruSeq Adapter, Index 11 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCGTCT1110.8117001828153565TruSeq Adapter, Index 11 (96% over 25bp)
CTCTTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCG890.6508226691042047RNA PCR Primer, Index 11 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCGTCTTC870.6361974405850092TruSeq Adapter, Index 11 (96% over 27bp)
TATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCGTCTT280.20475319926873858RNA PCR Primer, Index 11 (96% over 26bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3750.039.045
TCTCGTA4250.034.41176638
ATGCCGT4250.034.41176644
TCGTATG4250.034.41176640
GCATCTC4250.034.41176635
CTCGTAT4250.034.41176639
AGCCCAC4300.034.01162717
ACACGGC4300.034.01162727
GTATGCC4300.034.01162742
ATCTCGT4300.034.01162737
CCACGAG4300.034.01162720
TATGCCG4300.034.01162743
CATCTCG4300.034.01162736
GAGCCCA4300.034.01162716
CACGAGA4300.034.01162721
CCCACGA4300.034.01162719
GACACGG4300.034.01162726
CGTATGC4300.034.01162741
CGAGCCC4300.034.01162715
TCTCCGA4300.034.01162711
Produced by FastQC (version 0.11.2)