FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_89.3510000004727d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_89.3510000004727d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6440
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTC159024.689440993788818No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGT2584.0062111801242235No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCG1522.360248447204969No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1450.045.045
ATGCCGT1750.037.28571344
AGCCCAC1900.034.34210617
GTATGCC1900.034.34210642
TTCATCT1900.034.34210634
ATCTCGT1900.034.34210637
GCCCACG1900.034.34210618
CCACGAG1900.034.34210620
CATCTCG1900.034.34210636
GAGCCCA1900.034.34210616
CCCACGA1900.034.34210619
TCTCGTA1900.034.34210638
ACGAGAC1900.034.34210622
CGTATGC1900.034.34210641
TCGTATG1900.034.34210640
AGACCTA1900.034.34210625
CGAGACC1900.034.34210623
TCATCTC1900.034.34210635
GACCTAG1900.034.34210626
CTCGTAT1900.034.34210639
Produced by FastQC (version 0.11.2)