FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_88.35100000047247.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_88.35100000047247.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7753
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTC730.9415710047723461No Hit
GTGTAGGATAGGTGGGAGGCTTTGAAGCGTGGACGCTAGTTCACGTGGAGC280.36115052237843415No Hit
ATCCTACACAGATCGGTTCAAAGTCCAATGCAAAGCTACAGTAAAGGTTCA210.2708628917838256No Hit
GGTTTGTTTGAGGTTCTAACCTTGGCCCGTGAATCCGGGTCGGGGACAGTG200.2579646588417387No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTCT190.24506642589965175No Hit
GTGCATGGTAGGCAGTTTGACTGGGGCGGTCTCCTCCCAAAGTGTAACGGA140.18057526118921707No Hit
GCATTGGAGAAGGCGCTTAGGCAAATCCGGGCGCGGAATTCAAGGATGTGG140.18057526118921707No Hit
GGGTATGGCTGTTCGCCATTTAAAGAGGTACGTGAGCTGGGTTTAAAACGT110.14188056236295626No Hit
GCCTAGTGCTGCGAAGCAATTGGAAGTGGTTCCAAGAAAAGCCTCTAAGCT90.1160840964787824No Hit
GTAGGAGAGCGTTCTGTAAGCCTGTGAAGGTGTCTTGTAAAGGATGCTGGA90.1160840964787824No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTAC90.1160840964787824No Hit
GGTTAATATTCCTGCACCGTCGTATGATGCGATGGGGGGACGGATCGCGGA90.1160840964787824No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC90.1160840964787824No Hit
GTCGGGGACAGTGCATGGTAGGCAGTTTGACTGGGGCGGTCTCCTCCCAAA90.1160840964787824No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATG90.1160840964787824No Hit
GACGTATAGGGTGTGACGCCTGCCCGGTGCCGGAAGATTAAATGATGGGGT80.10318586353669547No Hit
GGTTAGAACCTCAAACAAACCAGGGTGGTATTTCAAGGACGGCTCCACGTG80.10318586353669547No Hit
GTGTTTGGCACCTCGATGTCGGCTCATCTCATCCTGGGGCTGTAGCCGGTC80.10318586353669547No Hit
GTTCGAAGGTACGCTTGGTACGGTCGGACATCGTACCTAAAGTGCAATGGC80.10318586353669547No Hit
GGTTTGTTTGAGGTTCTAACCTAGGTCCATTATCTGGATCGGGGACAGTGC80.10318586353669547No Hit
TAGCTACCCTGCGATGCCTCTGGCGAGACAACAGGTACACCAGCGGTACGT80.10318586353669547No Hit
TAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGAC80.10318586353669547No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)