Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0120_pe_85.351000000471f5.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 58897 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 39 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTC | 6615 | 11.231471891607383 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGT | 1052 | 1.786169074825543 | No Hit |
TTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTCT | 339 | 0.5755810992070903 | No Hit |
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 93 | 0.15790277942849382 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCG | 92 | 0.1562049000798003 | No Hit |
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 80 | 0.13583034789547854 | No Hit |
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA | 74 | 0.12564307180331766 | No Hit |
ATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTCTTC | 74 | 0.12564307180331766 | TruSeq Adapter, Index 9 (95% over 22bp) |
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 69 | 0.11715367505985025 | No Hit |
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA | 67 | 0.11375791636246328 | No Hit |
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA | 65 | 0.11036215766507632 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 740 | 0.0 | 41.959457 | 45 |
ATGCCGT | 885 | 0.0 | 34.83051 | 44 |
ATCTCGT | 900 | 0.0 | 34.250004 | 37 |
TCTCGTA | 900 | 0.0 | 34.250004 | 38 |
GATCTCG | 900 | 0.0 | 34.250004 | 36 |
CTCGTAT | 915 | 0.0 | 34.18033 | 39 |
CGTATGC | 925 | 0.0 | 34.054054 | 41 |
TCGTATG | 925 | 0.0 | 34.054054 | 40 |
CCGAGTT | 930 | 0.0 | 33.870968 | 28 |
AGACCGA | 930 | 0.0 | 33.870968 | 25 |
AGATCTC | 905 | 0.0 | 33.812157 | 35 |
GAGTTAG | 925 | 0.0 | 33.81081 | 30 |
ACGAGAC | 925 | 0.0 | 33.81081 | 22 |
TATGCCG | 920 | 0.0 | 33.750004 | 43 |
AGTTAGA | 920 | 0.0 | 33.750004 | 31 |
ACCGAGT | 935 | 0.0 | 33.68984 | 27 |
GTATGCC | 930 | 0.0 | 33.629032 | 42 |
CCACGAG | 930 | 0.0 | 33.629032 | 20 |
CACGAGA | 930 | 0.0 | 33.629032 | 21 |
GACCGAG | 930 | 0.0 | 33.629032 | 26 |