FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_85.351000000471f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_85.351000000471f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58897
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTC661511.231471891607383No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGT10521.786169074825543No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTCT3390.5755810992070903No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA930.15790277942849382No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCG920.1562049000798003No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.13583034789547854No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA740.12564307180331766No Hit
ATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTCTTC740.12564307180331766TruSeq Adapter, Index 9 (95% over 22bp)
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.11715367505985025No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA670.11375791636246328No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA650.11036215766507632No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7400.041.95945745
ATGCCGT8850.034.8305144
ATCTCGT9000.034.25000437
TCTCGTA9000.034.25000438
GATCTCG9000.034.25000436
CTCGTAT9150.034.1803339
CGTATGC9250.034.05405441
TCGTATG9250.034.05405440
CCGAGTT9300.033.87096828
AGACCGA9300.033.87096825
AGATCTC9050.033.81215735
GAGTTAG9250.033.8108130
ACGAGAC9250.033.8108122
TATGCCG9200.033.75000443
AGTTAGA9200.033.75000431
ACCGAGT9350.033.6898427
GTATGCC9300.033.62903242
CCACGAG9300.033.62903220
CACGAGA9300.033.62903221
GACCGAG9300.033.62903226