FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_84.351000000471db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_84.351000000471db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50558
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTC565311.18121761145615No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGT6681.3212547964713794No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTCT2880.5696427865026307No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.1305431385735195No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT580.11471972783733535No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.10878594881126627No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC540.10680802246924326No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA520.1028521697851972No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6350.041.81102445
GCCGTCT451.866465E-840.00000445
ATGCCGT7500.035.444
CGAGTTA7950.034.8113229
AGTTAGA8100.034.7222231
GAGTTAG8050.034.65838230
GATCTCG8000.034.5937536
GACCGAG8000.034.5937526
CCGAGTT8000.034.5937528
TTAGATC8000.034.5937533
ATCTCGT8050.034.3788837
GTTAGAT8050.034.3788832
GTATGCC7800.034.32692342
CACGAGA8000.034.312521
AGACCGA8000.034.312525
ACCGAGT8100.034.16666827
TCTCGTA8100.034.16666838
AGATCTC8100.034.16666835
TATGCCG7850.034.1082843
CCACGAG8050.034.09937720