FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_82.3510000004718b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_82.3510000004718b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84813
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTC57816.816172049096247No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGT7140.841852074564041No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2860.33721245563769703No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTCT2470.29122893895982926No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2420.2853336163088206No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2240.2641104547651893No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2140.2523198094631719No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA2060.24288729322155803No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1980.2334547769799441No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1890.22284319620812848No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA1560.1839340667114711No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1480.17450155046985721No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1480.17450155046985721No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA1410.16624809875844504No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA1320.1556365179866294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1290.1520993243960242No Hit
GATAGAAAGTGTTGCTTGTACTGATAGAAAGACAATCAGGAAAAGAAAGAA1250.14738306627521724No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA1220.14384587268461202No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1140.13441335644299812No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1080.1273389692617877No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA1050.12380177567118249No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1020.12026458208057728No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC1020.12026458208057728No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCG990.11672738848997205No Hit
ATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTCTTC930.10965300130876164TruSeq Adapter, Index 9 (95% over 22bp)
AGTGTACATAGCACCAATAAGTTTGTATCAACGGTTGTGAAATTTAAGACA860.10139954959734947No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTGTT253.860218E-545.0000047
AGTGTTG253.860218E-545.0000048
GTACTTC206.9963466E-445.036
TGCCGTC6550.039.8473345
GTGTTGC301.1310296E-437.5000049
TGTTGCT301.1310296E-437.50000410
ATGCCGT7100.036.4436644
ACAGTGA250.00209614536.00000410
TTTACTC250.00209614536.0000043
CAGTGAA250.00209614536.00000411
GCCGTCT702.0008883E-1135.35714345
CCCACGA7650.034.70588319
CGAGTTA7600.034.63815729
CACGAGA7600.034.63815721
GAGACCG7600.034.63815724
CCGAGTT7600.034.63815728
GAGTTAG7600.034.63815730
CCACGAG7600.034.63815720
CGTATGC7550.034.5695441
GACCGAG7550.034.5695426
Produced by FastQC (version 0.11.2)