FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_81.35100000047161.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_81.35100000047161.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25248
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTC726228.762674271229404No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGT6132.427915082382763No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTCT2330.9228453738910012No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCG1770.7010456273764258No Hit
ATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTCTTC1000.3960709759188847TruSeq Adapter, Index 9 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTCTT530.20991761723700886RNA PCR Primer, Index 9 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8600.041.84939245
ATGCCGT9600.037.4900844
CGAGCCC9900.037.03565615
CTCCGAG9900.037.03565612
AGCCCAC9950.036.8495417
ATCTCCG9950.036.8495410
ACATCTC9950.036.849548
CCGAGCC9950.036.8495414
TCTCCGA9950.036.8495411
CACGAGA9900.036.80844521
CCCACGA9900.036.80844519
ACGAGAC9900.036.80844522
AGACCGA9900.036.80844525
GAGACCG9900.036.80844524
GAGCCCA10000.036.665316
TCCGAGC10000.036.665313
ATCTCGT9950.036.6234737
CCACGAG9950.036.6234720
CGTATGC9950.036.6234741
TCGTATG9950.036.6234740