Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0120_pe_81.35100000047161.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 25248 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 50 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTC | 7262 | 28.762674271229404 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGT | 613 | 2.427915082382763 | No Hit |
TTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTCT | 233 | 0.9228453738910012 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCG | 177 | 0.7010456273764258 | No Hit |
ATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTCTTC | 100 | 0.3960709759188847 | TruSeq Adapter, Index 9 (95% over 22bp) |
TATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTCTT | 53 | 0.20991761723700886 | RNA PCR Primer, Index 9 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 860 | 0.0 | 41.849392 | 45 |
ATGCCGT | 960 | 0.0 | 37.49008 | 44 |
CGAGCCC | 990 | 0.0 | 37.035656 | 15 |
CTCCGAG | 990 | 0.0 | 37.035656 | 12 |
AGCCCAC | 995 | 0.0 | 36.84954 | 17 |
ATCTCCG | 995 | 0.0 | 36.84954 | 10 |
ACATCTC | 995 | 0.0 | 36.84954 | 8 |
CCGAGCC | 995 | 0.0 | 36.84954 | 14 |
TCTCCGA | 995 | 0.0 | 36.84954 | 11 |
CACGAGA | 990 | 0.0 | 36.808445 | 21 |
CCCACGA | 990 | 0.0 | 36.808445 | 19 |
ACGAGAC | 990 | 0.0 | 36.808445 | 22 |
AGACCGA | 990 | 0.0 | 36.808445 | 25 |
GAGACCG | 990 | 0.0 | 36.808445 | 24 |
GAGCCCA | 1000 | 0.0 | 36.6653 | 16 |
TCCGAGC | 1000 | 0.0 | 36.6653 | 13 |
ATCTCGT | 995 | 0.0 | 36.62347 | 37 |
CCACGAG | 995 | 0.0 | 36.62347 | 20 |
CGTATGC | 995 | 0.0 | 36.62347 | 41 |
TCGTATG | 995 | 0.0 | 36.62347 | 40 |