H23LNBCXX_l02n01_bir_0120_pe_80.35100000047148.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_0120_pe_80.35100000047148.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31017
Sequences flagged as poor quality	0
Sequence length	51
%GC	53

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTC	1793	5.780700905954799	RNA PCR Primer, Index 45 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTCT	1357	4.375020150240191	No Hit
ATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTCTTC	424	1.366992294548151	RNA PCR Primer, Index 45 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTCTT	187	0.6028951865106231	RNA PCR Primer, Index 45 (95% over 23bp)
GTGTAGGATAGGTGGGAGGCTTTGAAGCGTGGACGCTAGTTCACGTGGAGC	43	0.13863365251313794	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGT	42	0.13540961408259986	No Hit
GTGCATGGTAGGCAGTTTGACTGGGGCGGTCTCCTCCCAAAGTGTAACGGA	41	0.13218557565206177	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	41	0.13218557565206177	No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	39	0.12573749879098559	No Hit
CTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTCT	39	0.12573749879098559	No Hit
CTTATACACATCTCCGAGCCCACGAGCCCTTCCTTCATCTCGTATGCCGTC	37	0.11928942192990939	RNA PCR Primer, Index 45 (95% over 21bp)
GGGTATGGCTGTTCGCCATTTAAAGAGGTACGTGAGCTGGGTTTAAAACGT	32	0.10316922977721893	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATAACA	20	6.878508E-4	45.06462	4
TTATATA	20	6.878508E-4	45.06462	2
TACCGGA	20	6.933604E-4	44.991932	26
CTTATAC	410	0.0	33.52368	1
GCCGTCT	185	0.0	32.831955	45
CGTCTTC	35	2.760875E-4	32.137096	45
ACCGGAA	30	0.005075712	29.994625	27
CCGGAAG	45	3.3878106E-5	29.994625	28
AGACAGT	40	6.037086E-4	28.119957	6
TGCCGTC	505	0.0	27.618814	45
CTTCATC	515	0.0	27.519339	33
TTCATCT	515	0.0	27.519339	34
CCACGAG	515	0.0	27.519339	20
CCCACGA	515	0.0	27.519339	19
TCCTTCA	500	0.0	27.445082	31
ATGCCGT	500	0.0	27.445082	44
AGCCCAC	525	0.0	27.423655	17
ATCTCGT	510	0.0	27.34804	37
CCTTCAT	510	0.0	27.34804	32
CGTATGC	510	0.0	27.34804	41