FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_78.35100000047104.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_78.35100000047104.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244241
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTC2457710.062602102022183RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGT28801.1791632035571424No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA10630.43522586297959803No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7980.32672647098562485No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA6920.2833267141880356No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6390.26162683578924095No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6230.2550759291028124No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA6030.2468872957447767No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCG5960.24402127406946417No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5740.23501377737562493No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5150.21085730896941957No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4650.19038572557433028No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA4270.1748273221940624No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4210.1723707321866517No Hit
TTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTCT4180.17114243718294636No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA3960.16213494048910707No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA3670.15026142211995527No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3290.13470301873968743No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC3280.13429358707178565No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA2780.11382200367669637No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT2750.11259370867299101No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA2560.1048145069828571No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC206.997988E-445.031763
TGCCGTC27300.042.7698645
ATGCCGT29700.039.23796544
TATGCCG30300.038.4609743
ACGAGAC30550.038.440822
CACGAGA30850.038.35868521
CGTATGC30600.038.3044741
CATCTCG30450.038.2715136
CGAGACC30100.038.26807423
CTCGTAT30650.038.2419839
GTATGCC30500.038.20876742
GACCTTC30150.038.20461326
ACCTTCC30150.038.20461327
TCGTATG30700.038.179740
CCACGAG31000.038.17307720
GAGACCT30200.038.14135424
TCTCGTA30850.038.06698238
CCTTCCT30350.038.02697428
ATCTCGT30800.037.98269337
CCTTCAT30650.037.94837632
Produced by FastQC (version 0.11.2)