FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_77.351000000470e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_77.351000000470e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300765
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTC5101216.960750087277443RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGT211167.02076371918275No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCG24160.8032849566937642No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC23720.7886555948996725No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA17290.574867421408741No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC16510.5489335527737602No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA15980.5313118215217861No Hit
TTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTCT12100.40230744933752266No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA7830.2603361428357688No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC7450.24770169401359868No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA7080.2353997306867488No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA7050.234402274200788No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA6850.22775256429438265No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC6570.2184429704254152No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC6480.2154506009675328No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC6350.21112828952836935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA6150.204478579621964No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT6060.2014862101640816No Hit
GTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA5640.1875218193606304No Hit
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG4930.16391534919289147No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA4460.14828853091283895No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT4450.14795604541751867No Hit
AAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCC3960.13166425614682561No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT3910.13000182867022428No Hit
AAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACG3700.12301963326849866No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCT3550.11803235083869465No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACTGTCTCTTAT3340.11105015543696906No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTC3340.11105015543696906No Hit
GGTATCAACGCAGAGTACTTTTTTTTTCTGTCTCTTATACACATCTCCGAG3250.10805778597908666No Hit
GAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTC3210.1067278439978056No Hit
GGTATCAACGCAGAGTACTTTTTTTCTGTCTCTTATACACATCTCCGAGCC3210.1067278439978056No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC56050.043.15120745
GCCGTCT2150.041.858345
CGAGACG356.2351282E-638.56943523
CTGCACT301.1360382E-437.5105324
ACGCGAC250.00210517535.9981422
CACGCGA250.00210517535.9981421
CGAGACT504.8607035E-835.9981423
ATGCCGT77650.031.08982744
CCCACGC300.005142587729.9984519
CTTTGCG453.4835975E-529.998459
CCACGCG300.005142587729.9984520
TATGCCG81200.029.73060643
ACGAGAC79950.029.68889622
GTATGCC81350.029.67578542
CTCGTAT81300.029.66636339
CGAGACC78600.029.65495723
CGTATGC81550.029.63059441
CACGAGA80800.029.62718221
TCTCGTA81450.029.61172738
ATCTCGT81100.029.60081337
Produced by FastQC (version 0.11.2)