FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_74.3510000004708c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_74.3510000004708c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223028
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTC4686421.012608282368134RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGT141356.337769248704199No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCG25701.1523216815825816No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA11300.5066628405402013No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC11150.4999372276126764No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA10580.4743798984880822No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT9710.43537134350843837No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA9540.427748982190577No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC9020.4044335240418243No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA7340.32910665925354665No Hit
TTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTCT7040.31565543339849705No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT6670.29906558817726925No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA5950.26678264612515024No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT5900.26454077514930857No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC4710.2111842459242786No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC4610.20670050397259537No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTC4550.20401025880158546No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA4520.20266513621608048No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC4110.1842817942141794No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCT4080.18293667162867444No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT3940.17665943289631794No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTA3910.175314310310813No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC3890.17441756192047636No Hit
GAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTC3890.17441756192047636No Hit
GTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA3860.1730724393349714No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA3840.17217569094463475No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACTGTCTCTTAT3620.16231145865093172No Hit
AAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACG3530.15827609089441685No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTATA3500.15693096830891187No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTC3460.15513747152823862No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTAT2960.1327187617698226No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA2950.13227038757465429No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC2730.12240615528095126No Hit
GTATCAACGCAGAGTACGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA2660.11926753591477303No Hit
AAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCC2570.11523216815825815No Hit
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG2530.11343867137758488No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCCTTCATCTCGTATGCCGTC2460.11030005201140665RNA PCR Primer, Index 45 (95% over 21bp)
AAAAAGTACTCTGCGTTGATACCCCGTACCTGTCTCTTATACACATCTCCG2380.10671305845006009No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC2360.10581631005972345No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA2310.10357443908388185No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATC207.022944E-444.99753623
CAAGACA207.022944E-444.9975369
ACGTTCC302.1584237E-644.9975327
TGCCGTC51150.043.89788445
CGTTCCT356.2280105E-638.56931328
AGACGTT356.2280105E-638.56931325
GAGACGT356.2280105E-638.56931324
CGAGACA356.2280105E-638.56931323
CGAGACT601.546141E-1037.49794423
GAGACAT250.002104042335.99802824
CCAGTGC250.002104042335.99802822
ACGAGAT250.002104042335.99802822
GAGATCT250.002104042335.99802824
GCCGTCT1300.034.61348745
GACGTTC401.5555377E-533.74815426
CGAGACG401.5555377E-533.74815423
ATGCCGT67050.033.488144
GGGAGTA352.8140444E-432.14109419
GACTTTC708.076313E-1032.14109426
ACGAGAA352.8140444E-432.14109422
Produced by FastQC (version 0.11.2)