H23LNBCXX_l02n01_bir_0120_pe_73.35100000047062.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_0120_pe_73.35100000047062.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	136827
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTC	21362	15.612415678192171	RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGT	3042	2.223245412089719	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCG	703	0.5137874834645209	No Hit
TTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTCT	468	0.34203775570611067	No Hit
ATTCCACATTTCCTGTAGTTGATATGCAGGTTTTGTACAATATTGGGTTTC	201	0.14690083097634238	No Hit
GTATATGGATGAAAAGGTCTCCAGAGAAACATGTATCATAAAGCAAAGACA	196	0.1432465814495677	No Hit
TTTTTTCGTTGTGATATTCATTTTAGCACTATCTGAAATACGATTCGTTGC	191	0.13959233192279302	No Hit
CTTGTGTATCTAAACATAGTCTTACACTTTAATCCTGCTTGAGTTCAGACT	180	0.1315529829638887	No Hit
ATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTCTTC	172	0.1257061837210492	RNA PCR Primer, Index 45 (95% over 24bp)
TATCAACACCTTCTATTTGTCCAACTTTTGGTGTCAGTTAATGATGACATA	160	0.11693598485678995	No Hit
AAATGGAATGGAATGAAATGGAATGGAATGGTATTTCATTCCATTCCATTT	156	0.1140125852353702	No Hit
GTATAATGGTTGGAAACCAGTTATAGTTCTTCAAGTTTGCAAAGTAAATCA	152	0.11108918561395047	No Hit
GTACAAAGGAGGAATATGATAAAACCGAAAATGCAAAAAAAGAACGAGAGG	148	0.10816578599253071	No Hit
ATTACATTCAGGGGACCAACAAATGTGATTATCAACTGACTTCTTGTAAAA	147	0.10743493608717579	No Hit
CTACACATTTTATTTTTGAGGCTATCATAAAAGGGACTGAGTTATTGATTT	144	0.10524238637111095	No Hit
CATTCAATTCATTCGATGAAGATTCCATTCAATTCCGTTCAATGATTCCAT	139	0.10158813684433628	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTGACT	20	7.0114894E-4	45.000004	40
CTGTCAG	20	7.0114894E-4	45.000004	32
GGGTAGA	20	7.0114894E-4	45.000004	1
CGATTCC	20	7.0114894E-4	45.000004	30
TACTGGC	20	7.0114894E-4	45.000004	7
ATTGGAC	20	7.0114894E-4	45.000004	13
GGGGACC	25	3.8727503E-5	45.0	11
AAAACCG	35	1.2014607E-7	45.0	21
AGCCTAC	25	3.8727503E-5	45.0	16
GACCAAC	30	2.1514552E-6	44.999996	14
TGCCGTC	2265	0.0	42.019867	45
TGTGGTA	40	3.4285222E-7	39.375004	27
ACCGAAA	40	3.4285222E-7	39.375004	24
AAACCGA	40	3.4285222E-7	39.375004	22
CGAGAGG	40	3.4285222E-7	39.375004	45
ATAGGAC	35	6.2080253E-6	38.571426	3
GTGGTAC	35	6.2080253E-6	38.571426	28
ACGCATA	30	1.1346731E-4	37.499996	15
GGACTGA	30	1.1346731E-4	37.499996	34
CCAATGG	30	1.1346731E-4	37.499996	14