FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_72.35100000047049.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_72.35100000047049.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14216
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTC142210.002813731007315No Hit
TTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTCT2111.484243106359032RNA PCR Primer, Index 17 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGT510.35875070343275184No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA510.35875070343275184No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.3095104108047271No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.3024760832864378No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.3024760832864378No Hit
ATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTCTTC350.24620146314012378Illumina Paired End PCR Primer 2 (95% over 22bp)
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.19696117051209902No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA240.16882386043894204No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA220.15475520540236354No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA210.1477208778840743No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA200.14068655036578503No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.14068655036578503No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.13365222284749578No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA190.13365222284749578No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.13365222284749578No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.11958356781091727No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA170.11958356781091727No Hit
GTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA170.11958356781091727No Hit
TATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTCTT170.11958356781091727Illumina Paired End PCR Primer 2 (95% over 21bp)
GTGTAGGATAGGTGGGAGGCTTTGAAGCGTGGACGCTAGTTCACGTGGAGC160.11254924029262803No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT355.8239657E-638.57142645
ACGAGAC1850.035.2702722
CGAGACA1850.035.2702723
AGACACA1850.035.2702725
TGCCGTC1800.035.00000445
ATGCCGT1800.035.00000444
ATCTCGT1900.034.34210637
GCGATCT1900.034.34210634
CCACGAG1900.034.34210620
CACGAGA1900.034.34210621
CCCACGA1900.034.34210619
GACACAT1900.034.34210626
CGTATGC1900.034.34210641
GAGACAC1900.034.34210624
TAGCGAT1850.034.05405432
GTATGCC1850.034.05405442
CACATAG1850.034.05405428
TATGCCG1850.034.05405443
ACACATA1850.034.05405427
ATAGCGA1850.034.05405431
Produced by FastQC (version 0.11.2)