H23LNBCXX_l02n01_bir_0120_pe_71.3510000004702f.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_0120_pe_71.3510000004702f.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	55149
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTC	5447	9.876879000525848	No Hit
TCTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGT	787	1.4270431014161635	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	214	0.3880396743367967	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCG	151	0.2738036954432537	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	133	0.24116484433081292	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	124	0.22484541877459246	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	121	0.2194056102558523	No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	115	0.20852599321837204	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	103	0.18676675914341148	No Hit
TTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTCT	96	0.1740738725996845	RNA PCR Primer, Index 17 (95% over 21bp)
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC	95	0.17226060309343777	No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	93	0.16863406408094433	No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	80	0.14506156049973706	No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	76	0.13780848247475022	No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	76	0.13780848247475022	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	75	0.1359952129685035	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	73	0.13236867395601007	No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA	71	0.12874213494351666	No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC	59	0.1069829008685561	No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT	56	0.10154309234981596	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGTCT	35	1.1840166E-7	45.0	45
TGCCGTC	630	0.0	42.499996	45
CATAGCG	730	0.0	37.60274	30
GCGATCT	720	0.0	37.500004	34
AGCGATC	720	0.0	37.500004	33
ATGCCGT	715	0.0	37.44755	44
ATAGCGA	735	0.0	37.34694	31
ACATAGC	735	0.0	37.34694	29
TAGCGAT	730	0.0	37.29452	32
ACGAGAC	745	0.0	37.14765	22
CGAGACA	745	0.0	37.14765	23
GAGACAC	745	0.0	37.14765	24
CACATAG	740	0.0	37.094593	28
ATCTCGT	735	0.0	37.040817	37
TCTCGTA	735	0.0	37.040817	38
CGTATGC	735	0.0	37.040817	41
TCGTATG	735	0.0	37.040817	40
CTCGTAT	735	0.0	37.040817	39
CGATCTC	730	0.0	36.9863	35
GTATGCC	725	0.0	36.931034	42

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content