H23LNBCXX_l02n01_bir_0120_pe_69.35100000046fd0.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_0120_pe_69.35100000046fd0.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34880
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTC	11539	33.08199541284404	No Hit
TCTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGT	1673	4.79644495412844	No Hit
TTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTCT	299	0.8572247706422018	RNA PCR Primer, Index 17 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCG	186	0.533256880733945	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT	130	0.37270642201834864	No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA	105	0.30103211009174313	No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA	96	0.27522935779816515	No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT	88	0.2522935779816514	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA	85	0.24369266055045874	No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT	81	0.23222477064220184	No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC	81	0.23222477064220184	No Hit
CTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGGC	76	0.21788990825688076	No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA	64	0.1834862385321101	No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTA	60	0.17201834862385323	No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCT	59	0.169151376146789	No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTATA	57	0.16341743119266056	No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT	57	0.16341743119266056	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTC	56	0.16055045871559634	No Hit
CTTATACACATCTCCGAGCCCACGAGACACATAGCGATTTCGTATGCCGTC	48	0.13761467889908258	No Hit
GAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTC	45	0.12901376146788993	No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCT	44	0.1261467889908257	No Hit
CTTATACATATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTC	44	0.1261467889908257	No Hit
CTTATACACACTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTCT	42	0.12041284403669725	RNA PCR Primer, Index 17 (95% over 21bp)
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA	41	0.11754587155963303	No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC	40	0.11467889908256881	No Hit
ATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTCTTC	39	0.1118119266055046	Illumina Paired End PCR Primer 2 (95% over 22bp)
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCCTGTCTCT	38	0.10894495412844038	No Hit
CTTATACACATCTCCGAGCCCACGAGACACATAGCGATCCCGTATGCCGTC	38	0.10894495412844038	No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC	37	0.10607798165137615	No Hit
AAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTT	37	0.10607798165137615	No Hit
CTTATACACATCTCCGAGCCCACGAGACACCTAGCGATCTCGTATGCCGTC	37	0.10607798165137615	RNA PCR Primer, Index 38 (95% over 24bp)
TATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCTC	36	0.10321100917431193	No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACTGTCTCTTAT	35	0.10034403669724772	No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTAT	35	0.10034403669724772	No Hit
CTTATACACTCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTCT	35	0.10034403669724772	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTC	35	0.10034403669724772	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	1435	0.0	43.58885	45
CGGGTGG	40	3.3314063E-7	39.375	17
ATGCCGT	1705	0.0	36.554253	44
ACATAGC	1800	0.0	35.875	29
ACACATA	1815	0.0	35.826447	27
CGAGACA	1790	0.0	35.824024	23
CACATAG	1810	0.0	35.801105	28
GACACAT	1810	0.0	35.801105	26
AGCGATC	1785	0.0	35.79832	33
CATAGCG	1805	0.0	35.775623	30
GATCTCG	1780	0.0	35.772472	36
CGATCTC	1780	0.0	35.772472	35
ATAGCGA	1800	0.0	35.75	31
GCGATCT	1770	0.0	35.72034	34
GAGACAC	1815	0.0	35.70248	24
ATCTCGT	1785	0.0	35.672268	37
TCTCGTA	1780	0.0	35.646065	38
TAGCGAT	1795	0.0	35.59889	32
CCACGAG	1795	0.0	35.59889	20
CACGAGA	1795	0.0	35.59889	21

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content