FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_68.35100000046fb6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_68.35100000046fb6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293407
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTC246378.396868513702808No Hit
TCTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGT49811.69764184221917No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC9710.33093961630090624No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA8440.28765503208853227No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCG8290.2825426796225039No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC8210.27981609164062204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA6680.2276700964871322No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC5850.1993817461751083No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA5650.19256527622040373No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA5500.1874529237543753No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA5290.18029563030193554No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA5010.17075257236534916No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT4930.16802598438346733No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC4180.14246422205332526No Hit
TTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTCT4170.14212339855559003RNA PCR Primer, Index 17 (95% over 21bp)
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG4110.14007845756917867No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT3990.13598857559635594No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3770.1284904586461809No Hit
GGTATCAACGCAGAGTACTTTTTTTTTCTGTCTCTTATACACATCTCCGAG3400.11587998922997747No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC3110.10599610779565587No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGGCA253.8842038E-544.9989789
TGCCGTC28850.043.59519645
GCCGTCT800.042.18654345
ATGCCGT33550.037.4879744
CGTATGC35000.036.25631741
CTCGTAT35000.036.25631739
TCTCGTA35050.036.204638
TCGTATG35050.036.204640
ATCTCGT35000.036.19203637
GATCTCG34850.036.1541336
CGATCTC34800.036.1414235
GTATGCC34900.036.10233342
TATGCCG34950.036.05068643
GCGATCT34800.036.0121134
AGGATGG250.00210303236.0053185
GCATACC250.002104792535.9991824
AGCGATC34800.035.9474633
CGAGACA35100.035.8966223
TAGCGAT34850.035.89588532
ATAGCGA34850.035.89588531
Produced by FastQC (version 0.11.2)