FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_66.35100000046f73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_66.35100000046f73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146705
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTC5138635.026754371016665No Hit
TCTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGT1926313.130431818956408No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCG37732.5718278177294573No Hit
TTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTCT5670.386489894686616RNA PCR Primer, Index 17 (95% over 21bp)
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA2580.17586312668279883No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA2580.17586312668279883No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT2290.1560955659316315No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT1930.13155652499914794No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT1750.11928700453290617No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA1640.11178896424798065No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTA1640.11178896424798065No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC1590.10838076411846904No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC1580.10769912409256673No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCT1550.10565420401485975No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA1480.1008827238335435No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC55500.044.4692745
GCCGTCT702.0008883E-1135.35419545
ATGCCGT76250.032.36779444
CACGAGA80900.030.67420421
GCGATCT80650.030.65770534
ACGAGAC80950.030.65525822
GATCTCG80450.030.65002636
TCTCGTA80600.030.64881138
TATGCCG80750.030.647643
ATCTCGT80500.030.6309937
CCACGAG80950.030.62746420
CGATCTC80600.030.62089735
GACACAT80800.030.6007926
GTATGCC80900.030.59077542
AGCGATC80900.030.59077533
AGACACA81050.030.5896825
CTCGTAT80700.030.58295239
CATAGCG80950.030.5718830
TAGCGAT81000.030.5530132
CGTATGC81000.030.5530141
Produced by FastQC (version 0.11.2)