FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_65.35100000046f5a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_65.35100000046f5a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125788
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTC1350210.733933284574045No Hit
TCTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGT17251.371354978217318No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCG3850.306070531370242No Hit
TTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTCT3080.2448564250961936RNA PCR Primer, Index 17 (95% over 21bp)
ATGCTATAGATCATCTAGAGGCTGATTATGAAAGGAATGCACTTGAGAAAG2010.1597926670270614No Hit
GTCTAATATCCATAATCTACAAGTAACTCAAAGAAATCCACAACCAAAACA1780.14150793398416384No Hit
AATACAAAGAGCTAAACAAACAACAAAATGCTTAAATCACCACTTTTGCTT1670.13276306165929977No Hit
AACTAACATACACAAAGTCTGTGATGAAAATAAAACGGTTGTTATTCCTTT1610.12799313130028303No Hit
AACAACAACAAAGGTTTTGTTTTTTAGGCAACTGAAACTGCTGTAAAACAT1570.12481317772760517No Hit
ATACAGACATACACACAAATACATACACACACACACATGCACACATACACT1530.12163322415492733No Hit
TACATATGGAGAGAGAGATAAATGGATGGATGAACAAATCAGCCAAACAAT1440.11447832861640221No Hit
AGAGTAAACAATCCCAAAATAAAGAAGAGATGGTGACAGAACAATGTAGTT1390.1105033866505549No Hit
ATGTATGGTATGACACTAATAAAGATGGTATTCAAGGTGATGATGAAAAAG1340.1065284446847076No Hit
ATTTACTGTTAGTATGATGGTGGTTCCTTCATAAGTGACTAGATGCTTTTC1310.10414347950519923No Hit
CATAGAGGAAAGGAGAAAATGAAGTTAGCATTTGAACATCTTACAAGGGGA1300.10334849111202976No Hit
GAATCAAGTGGATCATCGAATAGAAACTAATGGAATCATTGTTGAAAGAAA1290.10255350271886031No Hit
GATGATATCTCTTGTAGCATACAAATCCATGGATTGTACATTCTTATTATC1290.10255350271886031No Hit
ACAATAAGGTCAATATCCTATTGAGAAACTTGCAGAAACATGCAAAGTATA1280.10175851432569084No Hit
CGTTTACTCTATGTTTTCTAGTTTGTGCTCATAGAGATGTTCATAGTAGTC1270.10096352593252139No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGAGA207.012675E-444.9956330
CTCAAGT207.012675E-444.9956325
TGCCAGG207.012675E-444.9956323
CAAGGGA207.012675E-444.9956321
CTGCCAG207.012675E-444.9956322
ATCCCAA207.012675E-444.9956311
CCCCCCT207.012675E-444.9956345
GCCTAAA207.012675E-444.9956312
GTCCCTA207.012675E-444.9956337
GGCTGTA207.012675E-444.9956321
GCTGTAT207.012675E-444.9956322
CCTGCCA207.012675E-444.9956321
TATAACT207.012675E-444.9956338
TCGAATC207.012675E-444.9956338
GCATAGA207.012675E-444.9956322
GCTGAGG253.8731756E-544.9956258
TCCCCCC253.8731756E-544.99562544
TCAAAGC253.8731756E-544.99562539
TGCCGTC14950.043.49075345
CCACAAC403.4281948E-739.37117438
Produced by FastQC (version 0.11.2)