H23LNBCXX_l02n01_bir_0120_pe_64.35100000046f30.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_0120_pe_64.35100000046f30.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	209897
Sequences flagged as poor quality	0
Sequence length	51
%GC	41

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCGTC	18333	8.734283958322415	No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCGTCT	1595	0.759896520674426	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCGT	994	0.47356560598769876	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	529	0.2520283758224272	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	518	0.24678771016260356	No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCGTCTTC	460	0.21915510941080624	RNA PCR Primer, Index 20 (95% over 23bp)
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	430	0.20486238488401456	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	423	0.20152741582776315	No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	412	0.1962867501679395	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	375	0.1786590565848964	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	355	0.16913057356703526	No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	310	0.14769148677684768	No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	283	0.13482803470273516	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	269	0.12815809659023233	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCG	253	0.12053531017594343	No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	233	0.1110068271580823	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	231	0.11005397885629618	No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCGTCTT	228	0.108624706403617	RNA PCR Primer, Index 20 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	211	0.10052549583843505	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	2135	40.4656	45
ATGCCGT	2205	39.180977	44
TATGCCG	2245	38.482876	43
CTCGTAT	2235	38.453728	39
GGTCATC	2210	38.379707	33
CGTATGC	2255	38.312218	41
GTATGCC	2255	38.21245	42
GTCATCT	2240	38.16701	34
TCTCGTA	2270	38.158165	38
TCGTATG	2265	38.14307	40
CCTGGTC	2250	38.097378	30
GAGACTC	2245	38.082012	24
ACGAGAC	2265	38.04374	22
ATCTCGT	2260	38.028355	37
AGACTCC	2245	37.981796	25
CTGGTCA	2245	37.981796	31
CACGAGA	2270	37.95994	21
GACTCCT	2245	37.88158	26
CCACGAG	2295	37.840534	20
TGGTCAT	2260	37.82925	32

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content