FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_63.35100000046f16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_63.35100000046f16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308316
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCGTC118523.8441079930979902No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCGT9410.3052063467351678No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA7750.2513654821676462No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCGTCT7300.23677006707404094No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4960.16087390858729356No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4350.14108901257151754No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4140.1342778188611684No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4140.1342778188611684No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA3970.128763995381362No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC3820.12389885701682689No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC3780.1226014867862842No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3280.10638435890450058No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCG3130.10151922053996548No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3100.10054619286705847No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGAC207.0244976E-445.00097319
GGCCTCT253.883647E-545.00097326
CTGAGTG207.030114E-444.9936759
GCACGGT207.030114E-444.99367537
CCGTTAT207.030114E-444.9936759
CGGATGT253.8873695E-544.9936741
TGCCGTC13000.041.1865245
ATGCCGT14150.037.83920744
GCCGTCT1100.036.81300745
TATGCCG14750.036.2999843
ATCTCGT15000.036.294937
CTCCTGG14700.036.27629528
TCTCGTA14900.036.23651538
CGTATGC14850.036.2070341
CTCGTAT14850.036.2070339
GTATGCC14800.036.17734542
CGAAACT250.002099476536.0183034
TGACCCA250.002099476536.0183034
TACGAGA250.002099476536.0183032
GGGTCTG250.002104499136.0007819
Produced by FastQC (version 0.11.2)