FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_61.35100000046ed1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_61.35100000046ed1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377145
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCGTC8491322.514682681727187No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCGT118073.13062615174535No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCG49731.3185909928542072No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT15560.4125734134086359No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT13510.35821766164207397No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT13260.3515889114266396No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG11800.31287701016850283No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA11540.30598310994445105No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT10370.2749605589362181No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTAT10240.2715136088241923No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG9690.25693035835023664No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA9410.24950615810895013No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC9310.24685465802277637No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA9170.24314255790213313No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTC8720.23121080751435122No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCT8710.23094565750573387No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA8470.22458205729891687No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA7790.20655185671293536No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA7370.19541555635100558No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTATA6570.17420355566161555No Hit
GCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC6570.17420355566161555No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCCTGTCTCT6400.16969600551512018No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCT6380.16916570549788543No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC6300.16704450542894642No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG6040.16015060520489466No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC5850.15511275504116454No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT5720.15166580492913867No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC5660.1500749048774344No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA5580.14795370480849543No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC5390.14291585464476528No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATAC5310.14079465457582627No Hit
AAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTT5180.1373477044638004No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATC5170.13708255445518303No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTA5160.13681740444656565No Hit
TACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTC5050.13390075435177454No Hit
GCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT5040.13363560434315713No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTC5040.13363560434315713No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCCTGTCTCTTA5010.132840154317305No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT4980.1320447042914529No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCCG4950.13124925426560075No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCTC4890.12965835421389652No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC4890.12965835421389652No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCTC4880.12939320420527914No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATC4800.12727200413634013No Hit
GATACCACCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA4720.12515080406740114No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCT4630.12276445398984476No Hit
GTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTC4570.1211735539381405No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG4450.117991753834732No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTA4410.1169311538002625No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA4400.11666600379164511No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCT4360.11560540375717561No Hit
GGTATCAACGCAGAGTACGGGTGGTATCCTGTCTCTTATACACATCTCCGA4340.11507510373994087No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATCT4330.1148099537313235No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATC4320.11454480372270612No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACA4300.11401450370547137No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTCTGTCTCTT4240.1124236036537671No Hit
TATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGAG4210.111628153627915No Hit
GAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTC4200.11136300361929762No Hit
GATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAG4190.11109785361068025No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCC4110.10897665354174124No Hit
GCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCC4110.10897665354174124No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC4100.10871150353312387No Hit
TGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACAT4090.1084463535245065No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATA4060.10765090349865436No Hit
GCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCG4020.10659030346418487No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCGTCT3930.10420395338662848No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATACA3860.10234790332630686No Hit
GTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCT3850.10208275331768947No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACCTGTCTC3790.10049185326598523No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGGT302.1622673E-644.99801344
TGCCGTC90950.044.30535545
ATGCCGT103550.038.9142644
TGCGGTC356.239021E-638.56972545
TATGCGG356.239021E-638.56972543
GCCGTCT1350.038.3316445
CATCTCG108500.037.2425936
GTCATCT108500.037.20112234
ATCTCGT108700.037.19476737
TATGCCG108450.037.15603643
GTATGCC108450.037.1352942
GGTCATC108950.037.1300733
TCTCGTA108900.037.1264638
CTCGTAT108850.037.1228439
TCGTATG109050.037.11665340
CGTATGC109200.037.10687641
TCATCTC108850.037.10217335
TGGTCAT109150.037.0826532
CTGGTCA109400.036.99790631
CCTGGTC109600.036.9714530
Produced by FastQC (version 0.11.2)