FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_57.35100000046e67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_57.35100000046e67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183880
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCGTC2537113.79758538177072No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCGT57863.1466173591472697No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCG9010.4899934740047857No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCGTCT6810.3703502284098325No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAATC253.8789578E-544.99809642
TGCCGTC26950.042.0761445
GTTAGTG301.1364789E-437.49841311
GCAGCTA301.1364789E-437.49841320
CTACTGC250.00210295435.99847818
ATTGACG250.00210295435.99847817
ATGCCGT33150.034.206744
TATGCCG34000.033.3515343
CGAGCCC34450.033.24242415
CCGAGCC34550.033.21132714
TCTCGTA34300.033.19101738
TCCGAGC34600.033.16333813
GTATGCC34200.033.1564942
AGCCCAC34550.033.1462117
GAGCCCA34500.033.12903216
ATCTCGT34400.033.0945337
CGTATGC34400.033.0945341
CTCGTAT34400.033.0945339
GCCCACG34550.033.0810918
ACGAGAC34400.033.02912522
Produced by FastQC (version 0.11.2)