FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_56.35100000046e4e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_56.35100000046e4e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90346
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGTC55396.130874637504704RNA PCR Primer, Index 33 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGTCT9551.0570473512939145RNA PCR Primer, Index 33 (100% over 21bp)
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4460.4936577158922365No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4360.48258915723994417No Hit
ATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGTCTTC4230.4682000309919642RNA PCR Primer, Index 33 (95% over 24bp)
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA4050.4482766254178381No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3780.4183915170566489No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3480.385185841099772No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA3070.3398047506253736No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2840.3143470657251013No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2760.30549221880326743No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2680.29663737188143363No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2590.28667566909437053No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2500.2767139663073075No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGT2240.2479357138113475No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2100.23243973169813828No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA1940.2147300378544706No Hit
TATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGTCTT1870.20698204679786597RNA PCR Primer, Index 33 (95% over 23bp)
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1660.18373807362805217No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1630.18041750603236445No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1600.1770969384366768No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA1590.17599008257144755No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1470.1627078121886968No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA1100.12175414517521528No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA1020.11289929825338144No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA910.10072388373585993No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCAC206.998772E-445.05
CGTCTTC504.7943104E-836.00000445
ATAGATG250.002096864636.0000043
TGCCGTC8900.034.38202345
CGAGACG9450.033.5714323
CCCACGA9500.033.39473719
AGACGGA9500.033.39473725
CTCGTAT9300.033.38709339
AGGCGAT9250.033.32432632
CACGAGA9550.033.21989421
GACGGAA9550.033.21989426
CCACGAG9550.033.21989420
CGGAAGG9650.033.10880728
GGAAGGC9650.033.10880729
GAGACGG9600.033.0468824
ACGAGAC9600.033.0468822
GCGATCT9200.033.01630834
TATGCCG9350.032.96791543
ATCTCGT9150.032.9508237
GCCCACG9650.032.8756518
Produced by FastQC (version 0.11.2)