H23LNBCXX_l02n01_bir_0120_pe_54.35100000046dfc.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_0120_pe_54.35100000046dfc.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	205984
Sequences flagged as poor quality	0
Sequence length	51
%GC	38

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGTC	17398	8.446287090259437	RNA PCR Primer, Index 33 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGT	1886	0.9156050955414012	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	1136	0.551499145564704	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	974	0.4728522603697375	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	851	0.41313888457355913	No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	825	0.4005165449743669	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	769	0.3733299673761069	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	733	0.3558528817772254	No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	726	0.35245455957744287	No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	596	0.28934286158148204	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	579	0.28108979338201023	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	521	0.25293226658381235	No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	494	0.23982445238465125	No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	481	0.23351328258505513	No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	446	0.21652167158614263	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCG	422	0.20487028118688833	No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA	406	0.19710268758738542	No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	317	0.1538954481901507	No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	307	0.1490407021904614	No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	304	0.1475842783905546	No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	302	0.14661332919061676	No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	284	0.137874786391176	No Hit
TTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGTCT	243	0.1179703277924499	RNA PCR Primer, Index 33 (100% over 21bp)
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT	231	0.11214463259282274	No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	229	0.11117368339288489	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	226	0.1097172595929781	No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	211	0.10243514059344416	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGTCT	45	3.8198777E-10	44.998787	45
TGCCGTC	1800	0.0	43.623825	45
ATGCCGT	1985	0.0	39.55813	44
TATGCCG	2035	0.0	38.586185	43
GGCGATC	2035	0.0	38.586185	33
TATATAC	35	6.214128E-6	38.579754	3
CGTATGC	2055	0.0	38.53911	41
CGGAAGG	2045	0.0	38.50752	28
GCGATCT	2040	0.0	38.491608	34
GAAGGCG	2040	0.0	38.491608	30
CTCGTAT	2060	0.0	38.445568	39
TCGTATG	2060	0.0	38.445568	40
ATCTCGT	2050	0.0	38.413597	37
GACGGAA	2050	0.0	38.413597	26
GTATGCC	2040	0.0	38.381317	42
GAGACGG	2065	0.0	38.352478	24
ACGAGAC	2065	0.0	38.352478	22
AGACGGA	2055	0.0	38.320137	25
AGGCGAT	2050	0.0	38.303844	32
GGAAGGC	2050	0.0	38.303844	29

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content