H23LNBCXX_l02n01_bir_0120_pe_52.35100000046db8.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_0120_pe_52.35100000046db8.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	220587
Sequences flagged as poor quality	0
Sequence length	51
%GC	38

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGTC	12073	5.473123982827637	RNA PCR Primer, Index 33 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGT	1130	0.5122695353760648	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	836	0.3789887890038851	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	613	0.27789488954471475	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	594	0.2692815079764447	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	582	0.26384147751227405	No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	578	0.2620281340242172	No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	566	0.2565881035600466	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	493	0.22349458490300878	No Hit
TTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGTCT	459	0.2080811652545254	RNA PCR Primer, Index 33 (100% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	424	0.19221440973402784	No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	422	0.1913077379899994	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	362	0.1641075856691464	No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	360	0.16320091392511799	No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	354	0.1604808986930327	No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	339	0.15368086061281944	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	337	0.152774188868791	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT	284	0.1287473876520375	No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA	243	0.11016061689945464	No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC	238	0.10789393753938355	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCG	238	0.10789393753938355	No Hit
ATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGTCTTC	226	0.10245390707521296	RNA PCR Primer, Index 33 (95% over 24bp)
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	224	0.10154723533118452	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA	224	0.10154723533118452	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCACTCG	20	7.021227E-4	44.99955	35
TCGGAGG	20	7.021227E-4	44.99955	39
CTCGGAG	20	7.021227E-4	44.99955	38
TGCCGTC	1505	0.0	41.860043	45
ATGCCGT	1635	0.0	38.531723	44
TCTCGTA	1665	0.0	37.972588	38
AGGCGAT	1660	0.0	37.951427	32
GCGATCT	1660	0.0	37.951427	34
ATCTCGT	1670	0.0	37.8589	37
GATCTCG	1665	0.0	37.837456	36
CGATCTC	1660	0.0	37.815884	35
GAGACGG	1685	0.0	37.788937	24
ACGAGAC	1685	0.0	37.788937	22
CGTATGC	1680	0.0	37.767475	41
CGAGACG	1680	0.0	37.767475	23
CTCGTAT	1675	0.0	37.745888	39
TATGCCG	1675	0.0	37.745888	43
AAGGCGA	1680	0.0	37.63355	31
CGGAAGG	1675	0.0	37.61156	28
GGCGATC	1675	0.0	37.61156	33

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content