FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_50.35100000046d75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_50.35100000046d75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155362
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGTC78235.0353368262509495RNA PCR Primer, Index 33 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGT15821.0182670151002176No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA5740.36945971344344175No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA5410.3482189982106307No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC4980.3205417026042404No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA4410.2838531944748394No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC4070.26196882120467035No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC3630.2336478675609222No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC3330.21433812644018488No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA3090.19889033354359495No Hit
TTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGTCT3070.19760301746887915RNA PCR Primer, Index 33 (100% over 21bp)
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA3060.1969593594315212No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTCTGTCTCTTATACACATCTCCG2860.18408619868436296No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA2830.18215522457228922No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2820.1815115665349313No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2800.18022425046021548No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCG2640.16992572186248892No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2550.1641327995262677No Hit
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG2530.16284548345155186No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2490.1602708513021202No Hit
GGTATCAACGCAGAGTACTTTTTTTTTCTGTCTCTTATACACATCTCCGAG2350.15125963877910945No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2220.14289208429345657No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT2160.13903013606930908No Hit
GTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGCC2080.1338808717704458No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2040.13130623962101415No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAG1950.12551331728479295No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA1910.12293868513536128No Hit
AAAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG1840.1184330788738559No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA1820.11714576279914007No Hit
AAAAAAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG1770.1139274726123505No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1760.11328381457499259No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTCTGTCTCTTATACACATCTCCGA1740.11199649850027676No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTCTGTCTCTTATACACATCTCCG1690.10877820831348721No Hit
AAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCC1680.1081345502761293No Hit
GTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGAG1650.10620357616405555No Hit
AAAAAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA1630.10491626008933973No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA1630.10491626008933973No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1580.10169796990255016No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACGG207.016172E-444.99774619
ATGCAGG207.016172E-444.99774626
GGTAGAG207.016172E-444.99774620
TGCCGTC9450.039.5218345
GGGCGGG301.1357179E-437.4981221
TGCAGGT250.002102032335.99819627
ATGCCGT11200.033.34654644
AACCTAT352.804754E-432.1515966
ACCTATG352.8100604E-432.141257
CGAGACG11550.032.14124723
GAGACGG11500.032.0853524
TATGCCG11650.032.05848343
CACGAGA11700.031.9214821
GGCGATC11700.031.9214833
ACGAGAC11800.031.84162522
AGACGGA11600.031.80875225
CGTATGC11750.031.78564341
AGGCGAT11750.031.78564332
GCGATCT11750.031.78564334
CCACGAG11750.031.78564320
Produced by FastQC (version 0.11.2)