FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_49.35100000046d5c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_49.35100000046d5c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135542
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGTC100027.379262516415576RNA PCR Primer, Index 33 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGT15181.1199480603798084No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCG3180.23461362529695592No Hit
TTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGTCT2890.213218043115787RNA PCR Primer, Index 33 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC11350.043.01550745
ATGCCGT13000.037.55584744
CGTATGC13500.036.33154341
CTCGTAT13500.036.33154339
CGAGACG13500.036.33154323
GCCCACG13450.036.29932818
AGCCCAC13450.036.29932817
TATGCCG13450.036.29932843
CCCACGA13450.036.29932819
GAGACGG13450.036.29932824
GATCTCG13450.036.29932836
CGATCTC13450.036.29932835
GCGATCT13350.036.23417334
GGCGATC13350.036.23417333
CGGAAGG13300.036.2012328
AGGCGAT13300.036.2012332
TCCGAGC13550.036.19748313
ACGAGAC13550.036.19748322
CCACGAG13550.036.19748320
AAGGCGA13250.036.16803431
Produced by FastQC (version 0.11.2)