FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_48.35100000046d32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_48.35100000046d32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154123
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGTC119897.778851955905348No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGT11900.7721105869986958No Hit
TTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGTCT6970.452236200956379Illumina PCR Primer Index 7 (95% over 21bp)
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3220.2089240411878824No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3050.1978938899450439No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2740.17778008473751486No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCG2570.16674993349467632No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC2560.166101101068627No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2530.16415460379047903No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA2380.15442211739973918No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2180.1414454688787527No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2040.13236181491406215No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2030.13171298248801283No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1860.12068283124517432No Hit
ATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGTCTTC1820.11808750154097701Illumina PCR Primer Index 7 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA1800.11678983668887835No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC1650.10705735029813851No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1650.10705735029813851No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1640.10640851787208919No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACAC351.2002056E-745.0123333
TGCCGTC13300.041.27610845
ATCCTAC356.187638E-638.594531
ATGCCGT14600.037.6008444
TATGCCG14850.036.9678343
GTATGCC14800.036.78868542
AGGTCAT14950.036.72055432
CACGAGA15050.036.62605721
CGTTAGG15050.036.62605728
ACGAGAC15050.036.62605722
CTCGTAT14900.036.5417839
CATCTCG14900.036.5417836
GACGTTA15100.036.5047826
CGAGACG15100.036.5047823
GTCATCT15050.036.47656234
GTTAGGT15050.036.47656229
ATCTCGT15000.036.44816237
TAGGTCA15000.036.44816231
GGTCATC15000.036.44816233
GAGACGT15150.036.38430424
Produced by FastQC (version 0.11.2)