FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_44.35100000046cc6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_44.35100000046cc6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282289
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGTC209927.4363506902500625No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGT35291.2501372706694203No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC8890.3149254841669353No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA8180.2897739550602397No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC7580.26851914173063773No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA7090.2511610441781295No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCG6730.23840815618036837No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA5560.1969612701876446No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC5210.18456262907871013No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA4770.16897576597033537No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA4740.16791302530385527No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4670.16543329708206836No Hit
TTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGTCT4660.16507905019324168Illumina PCR Primer Index 7 (95% over 21bp)
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG4300.1523261621954805No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4140.14665821197425333No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT4060.14382423686363974No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3980.14099026175302615No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC3930.13921902730889266No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA3890.13780203975358585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTCTGTCTCTTATACACATCTCCGAG3720.13177984264353199No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3720.13177984264353199No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA3680.1303628550882252No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3340.11831846086811744No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA3210.11371325131337034No Hit
AAAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG3210.11371325131337034No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT3030.10733680731448976No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3000.10627406664800966No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAG2970.10521132598152957No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACAC253.879873E-545.0067333
CCCCGTT253.883936E-544.9987645
TGCCGTC23150.042.8605945
ATGCCGT27150.036.5459544
TACCAGG250.00210287736.0053865
CTAGTAG250.002104706835.9990125
CGGGCGC250.002104706835.9990117
GGCATTC250.002104706835.9990141
GTATGCC27800.035.6105342
TATGCCG27900.035.56353443
TCGTATG28050.035.4535740
CGTTAGG28200.035.42455728
ATCTCGT28300.035.37888337
CGTATGC28050.035.3733641
CTCGTAT28150.035.3276239
GAGACGT28150.035.3276224
TAGGTCA28250.035.28221531
GACGTTA28300.035.2198826
GTTAGGT28300.035.2198829
AGACGTT28300.035.2198825
Produced by FastQC (version 0.11.2)