FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_43.35100000046cad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_43.35100000046cad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159441
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGTC2340514.679411192855039No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGT69294.34580816728445No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCG9600.6021035994505806No Hit
TTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGTCT4880.3060693297207117Illumina PCR Primer Index 7 (95% over 21bp)
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC2300.14425398736836822No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC2030.12731982363382066No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA1880.11791195489240533No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC1720.10787689490156234No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC1690.10599532115327928No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA1660.1041137474049962No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCAA207.0200564E-444.99341227
TGCCGTC25300.043.21501545
CGGGTAT504.840149E-835.9947316
ACGGGTA504.840149E-835.9947315
GCCGTCT1050.034.28069345
ATGCCGT32150.033.9374944
TCTCGTA33100.033.03141838
CATCTCG33000.032.99516736
ATCTCGT33150.032.98159437
TATGCCG33100.032.9634543
CTCGTAT33200.032.93192739
TCATCTC33250.032.81474335
CGAGACG33600.032.80769723
GTTAGGT33350.032.7838129
GAGACGT33700.032.77710324
CGTATGC33300.032.76547241
TCGTATG33300.032.76547240
ACGAGAC33650.032.7589522
ACGTTAG33400.032.7347327
GTATGCC33350.032.7163542
Produced by FastQC (version 0.11.2)