H23LNBCXX_l02n01_bir_0120_pe_42.35100000046c83.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_0120_pe_42.35100000046c83.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	116359
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGTC	40086	34.45027887829906	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGT	6773	5.82077879665518	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCG	2599	2.2336046201840856	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT	295	0.2535257264156619	No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC	275	0.2363375415739221	No Hit
TTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGTCT	265	0.2277434491530522	Illumina PCR Primer Index 7 (95% over 21bp)
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA	252	0.21657112900592135	No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG	236	0.20282058113252951	No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT	234	0.2011017626483555	No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT	209	0.1796165315961808	No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA	196	0.16844421144904992	No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA	185	0.15899070978609303	No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT	181	0.15555307281774508	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTC	178	0.15297484509148412	No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG	166	0.14266193418644024	No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTAT	162	0.1392242972180923	No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA	159	0.1366460694918313	No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCT	157	0.13492725100765735	No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT	140	0.12031729389217852	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCCTGTCTCT	140	0.12031729389217852	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTC	139	0.11945788465009154	No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTATA	130	0.11172320147130861	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTC	130	0.11172320147130861	No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCCTGTCT	129	0.11086379222922163	No Hit
AAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCTTA	127	0.10914497374504765	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA	126	0.10828556450296067	No Hit
AAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTT	119	0.10226969980835174	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCTT	20	7.007146E-4	45.000004	44
TTAATAC	20	7.007146E-4	45.000004	3
TAATACC	25	3.869155E-5	45.0	4
AATACCA	25	3.869155E-5	45.0	5
TGCCGTC	4245	0.0	43.67491	45
AAAATAC	35	6.2000145E-6	38.57143	1
GCCGTCT	120	0.0	37.500004	45
ATGCCGT	4970	0.0	37.303825	44
CGTTAAT	25	0.0020993508	36.0	1
CGTCTTC	25	0.0020993508	36.0	45
ACGAGAC	5275	0.0	35.445496	22
CATCTCG	5225	0.0	35.440193	36
ATCTCGT	5245	0.0	35.433746	37
TCTCGTA	5255	0.0	35.409134	38
TCGTATG	5245	0.0	35.390846	40
CCACGAG	5290	0.0	35.387524	20
CACGAGA	5290	0.0	35.387524	21
GTCATCT	5215	0.0	35.37872	34
CTCGTAT	5250	0.0	35.357143	39
TATGCCG	5245	0.0	35.34795	43

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content