FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_41.35100000046c69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_41.35100000046c69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146153
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGTC2391116.360252611988805No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGT72564.964660321717652No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCG9340.6390563313787606No Hit
TTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGTCT7970.545318946583375Illumina PCR Primer Index 7 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGTCTTC1960.13410603956128167Illumina PCR Primer Index 7 (95% over 23bp)
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC1530.10468481659630662No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC1500.10263217313363393No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC351.2022974E-744.99965745
TGCCGTC27250.042.1097745
TCTGAAC250.002101187835.99972530
AGATTAG250.002101187835.99972522
ATGCCGT34500.033.26061644
CTGTACA352.8085508E-432.1426124
CGTATGC36000.031.99975641
ATCTCGT35950.031.98167637
CATCTCG35900.031.96354536
TATGCCG35900.031.96354543
GGTCATC36050.031.95537833
GTATGCC35950.031.91908842
GAGACGT36100.031.91111424
CGAGACG36250.031.90320823
CTCGTAT36050.031.89296339
CGTTAGG36050.031.89296328
AGACGTT36050.031.89296325
TCTCGTA36050.031.89296338
AGGTCAT36200.031.88511532
ACGAGAC36350.031.87733822