Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0120_pe_41.35100000046c69.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 146153 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 40 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGTC | 23911 | 16.360252611988805 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGT | 7256 | 4.964660321717652 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCG | 934 | 0.6390563313787606 | No Hit |
TTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGTCT | 797 | 0.545318946583375 | Illumina PCR Primer Index 7 (95% over 21bp) |
ATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGTCTTC | 196 | 0.13410603956128167 | Illumina PCR Primer Index 7 (95% over 23bp) |
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC | 153 | 0.10468481659630662 | No Hit |
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC | 150 | 0.10263217313363393 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTCTTC | 35 | 1.2022974E-7 | 44.999657 | 45 |
TGCCGTC | 2725 | 0.0 | 42.10977 | 45 |
TCTGAAC | 25 | 0.0021011878 | 35.999725 | 30 |
AGATTAG | 25 | 0.0021011878 | 35.999725 | 22 |
ATGCCGT | 3450 | 0.0 | 33.260616 | 44 |
CTGTACA | 35 | 2.8085508E-4 | 32.142612 | 4 |
CGTATGC | 3600 | 0.0 | 31.999756 | 41 |
ATCTCGT | 3595 | 0.0 | 31.981676 | 37 |
CATCTCG | 3590 | 0.0 | 31.963545 | 36 |
TATGCCG | 3590 | 0.0 | 31.963545 | 43 |
GGTCATC | 3605 | 0.0 | 31.955378 | 33 |
GTATGCC | 3595 | 0.0 | 31.919088 | 42 |
GAGACGT | 3610 | 0.0 | 31.911114 | 24 |
CGAGACG | 3625 | 0.0 | 31.903208 | 23 |
CTCGTAT | 3605 | 0.0 | 31.892963 | 39 |
CGTTAGG | 3605 | 0.0 | 31.892963 | 28 |
AGACGTT | 3605 | 0.0 | 31.892963 | 25 |
TCTCGTA | 3605 | 0.0 | 31.892963 | 38 |
AGGTCAT | 3620 | 0.0 | 31.885115 | 32 |
ACGAGAC | 3635 | 0.0 | 31.877338 | 22 |