H23LNBCXX_l02n01_bir_0120_pe_39.35100000046c26.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_0120_pe_39.35100000046c26.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	244447
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGTC	24920	10.194438876320838	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGT	2949	1.2063964785822694	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	1159	0.4741314068080198	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1020	0.41726836492163943	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	865	0.35385993691884127	No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	836	0.3419964245828339	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	820	0.335451038466416	No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	733	0.2998605014583938	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	692	0.28308794953507305	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	578	0.2364520734555957	No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	551	0.22540673438414052	No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	548	0.22417947448731215	No Hit
TTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGTCT	539	0.22049769479682713	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	533	0.21804317500317044	No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	457	0.18695259095018552	No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	446	0.18245263799514824	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCG	436	0.17836177167238706	No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA	404	0.1652709994395513	No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	329	0.13458950201884254	No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	322	0.1317258955929097	No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	314	0.12845320253470077	No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	300	0.12272598968283513	No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	294	0.12027146988917845	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	289	0.11822603672779786	No Hit
ATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGTCTTC	282	0.11536243030186503	RNA PCR Primer, Index 24 (95% over 22bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGAGT	25	3.8819737E-5	44.99959	4
TGCCGTC	2620	0.0	42.766785	45
ATGCCGT	2950	0.0	37.982704	44
GGCATAG	2995	0.0	37.412014	29
TATGCCG	3000	0.0	37.34966	43
GTATGCC	3000	0.0	37.34966	42
CACGAGA	3025	0.0	37.338505	21
AGACGGC	3020	0.0	37.32582	25
CGTATGC	3015	0.0	37.313095	41
CGGCATA	3005	0.0	37.287514	28
GCATAGG	3005	0.0	37.287514	30
ACGAGAC	3030	0.0	37.27689	22
CATAGGA	2995	0.0	37.261765	31
TAGGATC	2995	0.0	37.261765	33
GAGACGG	3020	0.0	37.251316	24
ACGGCAT	3015	0.0	37.23847	27
GACGGCA	3015	0.0	37.23847	26
GGATCTC	2990	0.0	37.173576	35
CCACGAG	3040	0.0	37.15427	20
ATCTCGT	3010	0.0	37.150826	37

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content