FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_36.35100000046bc7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_36.35100000046bc7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255040
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGTC6121424.0017252195734No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGT227168.906838143036385No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCG20880.8186951066499373No Hit
TTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGTCT15670.614413425345044No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT8870.3477885821831869No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA7770.3046580928481807No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC6840.26819322459222084No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT6590.25839084065244666No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT6230.2442754077791719No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA6030.2364335006273526No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTC5080.19918444165621077No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCT5070.19879234629861983No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA4880.19134253450439148No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCCTGTCTCT4030.15801442910915933No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTATA4030.15801442910915933No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT3710.14546737766624845No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCG3620.14193851944792973No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTAT3180.12468632371392724No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCATA207.023857E-444.99882537
CTACCCC207.023857E-444.99882544
GGGCCAT207.023857E-444.99882511
GGTTGGG207.023857E-444.9988257
TGGGCCA253.8828417E-544.9988210
TGCCGTC66950.043.18408245
GCCGTCT2600.038.07592845
TACCCCT301.1376076E-437.49901645
TTGGGCC301.1376076E-437.4990169
TTTACCG250.002102286836.006125
GTTGGGC250.002104311735.9990588
CTTTATA606.379196E-933.7557372
ACTATGC606.3919288E-933.7491158
ATGCCGT90900.031.80609744
GCGTCCC501.9740382E-631.49917613
TGCGTCC501.9740382E-631.49917612
ATCTCGT94250.030.77107837
CGTATGC94550.030.7686341
TATGCCG94200.030.73964343
TCTCGTA94350.030.73846638
Produced by FastQC (version 0.11.2)