FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_35.35100000046bae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_35.35100000046bae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159434
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGTC2301714.436694807882885No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGT70664.431927945105811No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCG7020.44030758809287857No Hit
TTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGTCT5860.3675502088638559No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC2110.1323431639424464No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC2070.129834288796618No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA1820.11415381913519072No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC1810.11352660034873366No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA1810.11352660034873366No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC24100.042.56252745
TTGATGC250.002100887236.0027628
CGTCTTC250.002104126435.99146745
ACCTTAA250.002104126435.99146716
ATGCCGT31650.032.40937844
CTCGTAT33200.031.23506539
TCTCGTA33250.031.19788238
ATCTCGT33200.031.17709237
GATCTCG33150.031.15623336
TATGCCG33150.031.14646143
CGTATGC33300.031.14126641
GTATGCC33200.031.09955642
TCGTATG33350.031.09457640
GGATCTC33250.031.06253235
GCATAGG33350.031.03686130
AGACGGC33300.031.01589225
ACGGCAT33300.031.01589227
GGCATAG33450.031.01134729
GACGGCA33250.030.99485826
CGGCATA33400.030.99040228