FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_34.35100000046b84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_34.35100000046b84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74547
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGTC1482819.89080714180316No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGT59637.998980508940669No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCG5000.6707178021919058No Hit
TTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGTCT4420.5929145371376447No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCG1170.15694796571290595No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA1110.14889935208660307No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC970.13011925362522972No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC970.13011925362522972No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA950.1274363824164621No Hit
ATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGTCTTC850.11402202637262399RNA PCR Primer, Index 24 (95% over 22bp)
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC830.11133915516385637No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC830.11133915516385637No Hit
TATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGTCTT780.1046319771419373RNA PCR Primer, Index 24 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC15100.042.75788545
CCGTCTT250.002096235.9940945
TCATGGC250.002096235.9940910
GCCGTCT754.5474735E-1132.99458745
ATGCCGT21200.030.45490844
ATCTCGT21800.029.73985337
TCTCGTA21800.029.73985338
CTCGTAT21800.029.71989439
GGATCTC21750.029.7047235
GATCTCG21750.029.7047236
TATGCCG21750.029.68478443
CCACGAG22000.029.5519720
CCCACGA22100.029.52004419
ACGAGAC22050.029.48495922
GTATGCC21900.029.48146242
AGACGGC21900.029.48146225
CGTATGC21900.029.48146241
AGGATCT21950.029.43406334
CGGCATA21950.029.43406328
CACGAGA22100.029.41825121
Produced by FastQC (version 0.11.2)