FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0120_pe_33.35100000046b5e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0120_pe_33.35100000046b5e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117984
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGTC4047634.30634662327095No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGT1532412.988201790073232No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCG13921.1798209926769732No Hit
TTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGTCT7960.6746677515595335No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA1930.16358150257662057No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCG1870.15849606726335774No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA1760.14917276918904257No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA1730.14663005153241118No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCT1340.11357472199620287No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT1210.10255627881746678No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC1210.10255627881746678No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC1190.10086113371304582No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC42850.043.62778545
GCCGTCT1300.039.80131545
TGTACTC250.002088026636.040075
TTGTACT352.7868882E-432.178634
TTTGTAC352.7868882E-432.178633
ATGCCGT60150.031.0798144
ACGAGAC62400.030.24755922
GACGGCA62200.030.23631526
ACGGCAT62200.030.23631527
GAGACGG62350.030.23573524
CACGAGA62500.030.23515521
GCATAGG61850.030.22555530
AGACGGC62250.030.2120325
CCACGAG62550.030.21098920
CGGCATA62200.030.20014628
CCCACGA62500.030.19916219
CGAGACG62500.030.19916223
GGATCTC61800.030.17720635
TAGGATC61950.030.17676433
GGCATAG62050.030.16438929
Produced by FastQC (version 0.11.2)